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Flaxseed Globulin Isolation and its Evaluation for Hypocholesterolemic Effect by In Vivo Studies.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/11931/
 
Title Flaxseed Globulin Isolation and its
Evaluation for Hypocholesterolemic Effect by In Vivo Studies.
 
Creator Lija, P. J.
 
Subject 04 Diabetes Mellitus
03 Proteins
01 Oilseeds
 
Description Here flaxseed, a storage protein has been fractionated into albumin, globulin, glutelin and prolamine. Fractionation was carried out from defatted dehulled flaxseed meal by sequential solvent extraction. Globulin and albumin fractions were the predominant protein fractions accounting for 47.3 % and 37.8 % respectively, while the glutelin and prolamin accounted for 16.7 % and 9 % respectively. The current study is focused on isolation of major fraction of flaxseed protein (globulin isolate) with high protein content and its evaluation for cholesterol lowering effect in hypercholesterolemic induced rats. The maximum extractability (51.45 %) of flaxseed globulin was showed at 3 % sodium chloride (NaCl) concentration. Flaxseed globulin showed maximum solubility at pH 2 and the isolelectric point of globulin was at pH 5. The globulin isolate prepared had a protein content of 93 %. SDS PAGE patterns of the globulin isolate showed three bands at 36.5 kDa, 26.6 kDa and
 
Contributor Purnima Kaul, Tiku
 
Date 2015
 
Type Student Project Report
NonPeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/11931/1/lija.pdf
Lija, P. J. (2015) Flaxseed Globulin Isolation and its Evaluation for Hypocholesterolemic Effect by In Vivo Studies. [Student Project Report] (Submitted)