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Evaluation of Various Culture Conditions for the Production of Poly Unsaturated Fatty Acids (PUFAs) from selected Marine Micro-Algae.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/11941/
 
Title Evaluation of Various Culture Conditions for the Production of
Poly Unsaturated Fatty Acids (PUFAs) from selected Marine
Micro-Algae.
 
Creator Parul, Yadav
 
Subject 17 Fatty Acid Chemistry
01 Algae
 
Description Polyunsaturated fatty acids (PUFAs) have been associated with various health benefits and
are commonly obtained from fish oil. However continuous exploitation of fish biodiversity
for PUFA rich oil would create ecological imbalance. Hence identifying alternative sources
for production of PUFA rich oil is necessary. Marine microalgae can be a targeted as
vegetarian source of PUFAs since they are primary producers of aquatic ecosystem and
marine organisms like fish feed on microalgae for meeting their nutritional requirements.
Hence towards this direction, two marine microalgal species belonging to the genus of
Tetraselmis sp. (Tetraselmistheli and Tetraselmischuii) were evaluated as source of omega 3
PUFA mainly Eicosapentaenoic acid (C-20:5). The selected microalgal strains were
maintained at CSIR-CFTRI algal culture facility. The strains were evaluated for their growth,
biomass accumulation and lipid profile under different cultivation parameters such as light
intensity and photo-period, carbon supplementation (sodium acetate), incubation temperature
and salinity levels. High light intensity at 60 μE m−2 s−1 increased growth and biomass
concentration and lipid content in both the strains, however the EPA content decreased by 2
folds at higher light intensity. Under colder incubation conditions (15°C) the total lipid
content EPA content increased by 1.5 to 2 folds, without affecting the biomass concentration.
Maximum growth and biomass concentration was observed at 25mM acetate supplementation
for both the strains. However with increasing acetate levels the total lipid and EPA content
decreased by 2-3 folds. Both the strains could tolerate high salinity (1M NaCl), however
showed a reduced growth and biomass concentration. The monounsaturated fatty acid levels
such as palmito-oleic acid (C-16:1) and oleic acid (C-18:1) increased with increasing salinity,
while EPA content did not show any significant variations. From this study it is evident that
the Tetraselmis sp. could tolerate stressful culture conditions such as colder incubation
temperature and high salt concentration and could be exploited as potential source of EPA for
nutritional applications.
 
Contributor Chauhan, V. S.
 
Date 2015
 
Type Student Project Report
NonPeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/11941/1/parul.pdf
Parul, Yadav (2015) Evaluation of Various Culture Conditions for the Production of Poly Unsaturated Fatty Acids (PUFAs) from selected Marine Micro-Algae. [Student Project Report] (Submitted)