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Extracellular phytase from Aspergillus niger CFR 335: purification and characterization.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/12026/
http://dx.doi.org/10.1007/s13197-014-1304-z
 
Title Extracellular phytase from Aspergillus niger CFR 335:
purification and characterization.
 
Creator Gunashree, B. S.
Venkateswaran, G.
 
Subject 04 Fungi
05 Enzymes
 
Description Phytase, that is extensively used as a feed additive
is capable of hydrolyzing phytic acid, an antinutrient found in
about 60–80 % of all the plant commodities. This enzyme
improves the bioavailability of essential minerals such as
Ca2+, Mg2+, P, Zn2+, Fe3+, that are bound to phytic acid. An
extracellular phytase from a local fungal isolate, Aspergillus
niger CFR 335 was purified to homogeneity through a threestep
column chromatography using DEAE-Sephadex anion
exchanger. An active fraction of the enzyme was obtained
with NaCl gradient of 2.5Min DEAE Sephadex column. The
enzyme was purified up to 16 fold with a yield of 28.5 %.
Substrate specificity studies revealed a highest specific activity
of 32.6±3.1 U/mg for sodium phytate with the Km value of
0.08±0.1 mM. The molecular weight of the enzyme was
66 kDa with an optimum temperature of 30 °C and pH 4.5.
Up to 80 % of the activity was retained even after storing the
enzyme for 6 months at 4 °C.
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/12026/1/Journal%20of%20Food%20Science%20and%20Technology%20July%202015%2C%20Volume%2052%2C%20Issue%207%2C%20pp%204558-4564.pdf
Gunashree, B. S. and Venkateswaran, G. (2015) Extracellular phytase from Aspergillus niger CFR 335: purification and characterization. Journal of Food Science and Technology, 52 (7). pp. 4558-4564.