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Role of different vehicles in carotenoids delivery and their influence on cell viability, cell cycle progression, and induction of apoptosis in HeLa cells.

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Relation http://ir.cftri.com/12031/
http://dx.doi.org/10.1007/s11010-015-2442-y
 
Title Role of different vehicles in carotenoids delivery and their
influence on cell viability, cell cycle progression, and induction
of apoptosis in HeLa cells.
 
Creator Sowmya, Poorigali Raghavendra
Arathi, Bangalore Prabhashankar
Vijay, K.
Baskaran, V.
Lakshminarayana, Rangaswamy
 
Subject 14 Carotenoid Chemistry
 
Description The objective of the present study was to determine
the role of different vehicles in carotenoids delivery
and their influence on cell viability, cell cycle
progression and induction of apoptosis in HeLa cells. Cells
(5 9 103) were treated with different concentrations
(25–100 lM) of b-carotene (BC) or lutein (L) or astaxanthin
(AST) dissolved in 0.5 % of tetrahydrofuran (THF),
dimethylsulfoxide (DMSO), and fetal bovine serum (FBS),
respectively. The effect of delivery vehicle on carotenoids
uptake, cytotoxicity, oxidative status, cell cycle distribution,
and apoptosis was examined after 48 h of incubation.
The results shown that, cell viability reduced significantly
in a dose- and time-dependent manner irrespective of carotenoid
delivered in vehicles. Cellular uptake of BC delivered
in THF was higher by 49.1, 29.7 % and L delivered
through THF was higher by 41.7 and 37.5 % than DMSO
and FBS, respectively. While, AST delivered through
DMSO was higher by 36.1 and 43.7 % than the THF and
FBS, respectively. In case of cells treated either with BC or
L delivered through THF and AST in DMSO decreased the
glutathione and increased the malondialdehyde levels. The
net increase in the G2/M phase percentage of cell cycle
progression was observed in carotenoid-treated cells. The
% induction of apoptosis by BC or L delivered with THF
and AST in DMSO was higher than other treated groups. In
conclusion, choice of suitable vehicle for specific
carotenoids delivery is essential that in turn may influence
on cell proliferation and cell-based assays.
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12031/1/Molecular%20and%20Cellular%20Biochemistry%20August%202015%2C%20Volume%20406%2C%20Issue%201%2C%20pp%20245-253.pdf
Sowmya, Poorigali Raghavendra and Arathi, Bangalore Prabhashankar and Vijay, K. and Baskaran, V. and Lakshminarayana, Rangaswamy (2015) Role of different vehicles in carotenoids delivery and their influence on cell viability, cell cycle progression, and induction of apoptosis in HeLa cells. Molecular and Cellular Biochemistry, 406 (1). pp. 245-253.