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Antiproliferative, ACE-inhibitory and functional properties of protein hydrolysates from rohu (Labeo rohita) roe (egg) prepared by gastrointestinal proteases.

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Relation http://ir.cftri.com/12066/
http://dx.doi.org/10.1007/s13197-015-1969-y
 
Title Antiproliferative, ACE-inhibitory and functional properties
of protein hydrolysates from rohu (Labeo rohita) roe (egg)
prepared by gastrointestinal proteases.
 
Creator Chalamaiah, M.
Jyothirmayi, T.
Prakash, V. Diwan
Dinesh Kumar, B.
 
Subject 03 Proteins
Fish
 
Description Previously, we have reported the chemical composition,
molecular mass distribution and antioxidant activity of
rohu roe protein hydrolysates. In the current study, antiproliferative,
angiotensin-converting enzyme (ACE)-inhibitory activities
and functional properties of protein hydrolysates from
rohu (Labeo rohita) roe proteins, prepared by gastrointestinal
proteases (pepsin and trypsin), were investigated.
Antiproliferative activity was evaluated against human colon
cancer cell line Caco-2. The results showed that the pepsin
hydrolysate possessed dose dependent inhibitory effect on
Caco-2 cell line. Pepsin and trypsin hydrolysates displayed
ACE-inhibitory activity in vitro. The ACE-inhibitory activity
of the hydrolysate generated by pepsin (47 ± 1.7 %, at
1 mg/ml) is higher than that obtained by trypsin
(36 ± 3.2 %). Additionally, the undigested rohu roe proteins
and its hydrolysates exhibited functional properties.
Solubilities of the hydrolysates were above 81 ± 9.2 % at all
pH values tested. Pepsin and trypsin hydrolysates showed
good foaming capacity (45–211 %) and emulsification activity
(4–29 m2/g). The foaming abilities and emulsifying activity
index (EAI) were affected by pH. The results suggest that
protein hydrolysates from rohu roe could be useful in food
industry for various applications.
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12066/1/Journal%20of%20Food%20Science%20and%20Technology%20December%202015%2C%20Volume%2052%2C%20Issue%2012%2C%20pp%208300-8307.pdf
Chalamaiah, M. and Jyothirmayi, T. and Prakash, V. Diwan and Dinesh Kumar, B. (2015) Antiproliferative, ACE-inhibitory and functional properties of protein hydrolysates from rohu (Labeo rohita) roe (egg) prepared by gastrointestinal proteases. Journal of Food Science and Technology, 52 (12). pp. 8300-8307. ISSN 0022-1155