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Comparative study on characteristics and in vitro fibril formation ability of acid and pepsin soluble collagen from the skin of catla (Catla catla) and rohu (Labeo rohita).

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Relation http://ir.cftri.com/12073/
http://dx.doi.org/10.1016/j.foodres.2015.07.018
 
Title Comparative study on characteristics and in vitro fibril formation ability
of acid and pepsin soluble collagen from the skin of catla (Catla catla)
and rohu (Labeo rohita).
 
Creator Gaurav Kumar, Pal
Nidheesh, T.
Suresh, P. V.
 
Subject 03 Proteins
Fish
 
Description Catla (Catla catla) and Rohu (Labeo rohita) are the major carp fish produced by freshwater aquaculture in India.
Processing of these carp fish generates potentially large quantities of by-products (waste) from non-edible fish
parts by fish-processing factories and fish shops. The paper focuses on the extraction of the acid soluble collagen
(ASC) and pepsin soluble collagen (PSC) from the skin of carp fish and subsequently their characteristics and in
vitro fibril-forming ability. The extracted collagens are characterized as type I collagen based on the electrophoretic
pattern. The denaturation temperature for all the collagens extractedwas found to be 30.69–35.19 °C by differential
scanning calorimetry (DSC) analysis. The extracted collagens exhibited higher solubility at low
concentration of NaCl (0–0.4 M). All the ASC and PSC displayed different degrees of fibril-forming abilities and
the scanning electron microscopy (SEM) analysis confirmed their well-defined fibril morphologies. The degree
of collagen fibril formationwas significantly (p ≤ 0.05) higher (78%) in rohu skin collagen than catla skin collagen
(36%). In general, the characteristics of two carp skin collagenswere unique as evidenced by the electrophoretic,
DSC, SEMand fibril-forming patterns. Overall, the results indicated the feasibility of using the carp skin as a good
alternative source of realistic high-quality collagen.
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12073/1/Food%20Research%20International%2C%20Volume%2076%2C%20Part%203%2C%20October%202015%2C%20Pages%20804-812.pdf
Gaurav Kumar, Pal and Nidheesh, T. and Suresh, P. V. (2015) Comparative study on characteristics and in vitro fibril formation ability of acid and pepsin soluble collagen from the skin of catla (Catla catla) and rohu (Labeo rohita). Food Research International, 76 (3). pp. 804-812.