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Antioxidant and neuroprotective potential of chitooligomers inCaenorhabditis elegans exposed to Monocrotophos.

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Relation http://ir.cftri.com/12084/
http://dx.doi.org/10.1016/j.carbpol.2015.08.055
 
Title Antioxidant and neuroprotective potential of chitooligomers inCaenorhabditis elegans exposed to Monocrotophos.
 
Creator Nidheesh, T.
Chinnu, Salim
Rajini, P. S.
Suresh, P. V.
 
Subject 17 Toxicology
Fish
32 Antioxidants
 
Description tThe objectives of this investigation were to establish the propensity of the chitooligomers (COS) to amelio-rate neurodegeneration and oxidative stress in Caenorhabditis elegans induced by an organophosphorusinsecticide, Monocrotophos (MCP). COS was prepared from �-chitosan by the enzymatic method usingchitosanase and characterized by HPLC and electron spray ionization-TOF-(ESI-TOF)-MS. We exposedage synchronized L4 C. elegans worms (both wild type N2 and transgenic strain BZ555 (Pdat-1:GFP) tosublethal concentration of MCP (0.75 mM) for 24 h in the presence or absence of COS (0.2 mM). Theneuroprotective effect of COS was examined in N2 worms in terms of brood size, lifespan, egg laying,dopamine content, acetylcholinesterase and carboxylesterase activity and by direct visualization andquantification of degeneration of dopaminergic neurons in BZ555. Exposure to COS extended lifespan,normalized egg laying, increased brood size, decreased the dopaminergic neurodegeneration, increasedthe dopamine content and increased AChE and carboxylesterase activity in C. elegans treated with MCP.COS induced a significant decrease in reactive oxygen species and increased the reduced glutathione levelas well as increased superoxide dismutase and catalase activity. Our findings demonstrate that COS sig-nificantly inhibits the dopaminergic neurodegeneration and associated physiological alterations inducedby MCP in C. elegans by attenuating the oxidative stress as well.
 
Date 2016
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12084/1/Carbohydrate%20Polymers%2C%20Volume%20135%2C%201%20January%202016%2C%20Pages%20138-144.pdf
Nidheesh, T. and Chinnu, Salim and Rajini, P. S. and Suresh, P. V. (2016) Antioxidant and neuroprotective potential of chitooligomers inCaenorhabditis elegans exposed to Monocrotophos. Carbohydrate Polymers, 135 (1). pp. 138-144.