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Application of a molecular beacon based real-time isothermal amplification (MBRTIA) technology for simultaneous detection of Bacillus cereus and Staphylococcus aureus

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Relation http://ir.cftri.com/12095/
http://dx.doi.org/10.1007/s13197-014-1525-1
 
Title Application of a molecular beacon based real-time
isothermal amplification (MBRTIA) technology for simultaneous
detection of Bacillus cereus and Staphylococcus aureus
 
Creator Mandappa, I. M.
Prasanna, Joglekar
Manonmani, H. K.
 
Subject 10 Food Microorganisms
 
Description A multiplex real-time isothermal amplification assay
was developed using molecular beacons for the detection
of Bacillus cereus and Staphylococcus aureus by targeting
four important virulence genes. A correlation between
targeting highly accessible DNA sequences and isothermal
amplification based molecular beacon efficiency and sensitivity
was demonstrated using phi(Φ)29 DNA polymerase at a
constant isothermal temperature of 30 °C. It was very selective
and consistently detected down to 101 copies of DNA. The
specificity and sensitivity of this assay, when tested with pure
culture were high, surpassing those of currently used PCR
assays for the detection of these organisms. The molecular
beacon based real-time isothermal amplification (MBRTIA)
assay could be carried out entirely in 96 well plates or well
strips, enabling a rapid and high-throughput detection of food
borne pathogens.
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12095/1/Journal%20of%20Food%20Science%20and%20Technology%20July%202015%2C%20Volume%2052%2C%20Issue%207%2C%20pp%204642-4646.pdf
Mandappa, I. M. and Prasanna, Joglekar and Manonmani, H. K. (2015) Application of a molecular beacon based real-time isothermal amplification (MBRTIA) technology for simultaneous detection of Bacillus cereus and Staphylococcus aureus. Journal of Food Science and Technology, 52 (7). pp. 4642-4646.