ICAR Research Data Repository for Knowledge Management
Transgalactosylating β-galactosidase from probiotic Lactobacillus plantarum MCC2156: production and permeabilization for use as whole cell biocatalyst
IR@CSIR-CFTRI
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/12097/
http://dx.doi.org/10.1007/s13197-014-1656-4 |
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| Title |
Transgalactosylating β-galactosidase from probiotic Lactobacillus plantarum MCC2156: production and permeabilization for use as whole cell biocatalyst |
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| Creator |
Gobinath, D.
Prapulla, S. G. |
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| Subject |
05 Enzymes
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| Description |
Key nutritional factors were optimized for the maximum production of transgalactosylating β-galactosidase from Lactobacillus plantarum MCC2156. Galactose, yeast extract, sodium acetate and manganese sulphate were the most important nutrients affecting β-galactosidase production. Maximum β-galactosidase production (3015 miller units) was obtained by culturing L. plantarum in the optimized fermentation medium containing (w/v) galactose (4 %), yeast extract (2 %), sodium acetate (3 %) and manganese sulphate (0.075 %) with an optimum medium pH of 7.0, after 14 h of incubation at 35 °C. Further, permeabilization of L. plantarum cells using various chemical/ solvents for maximum β- galactosidase activity was performed for use as whole cell biocatalyst. Mixture of ethanol: n-butanol was found to effectively permeabilize the cells with maximum β-galactosidase activity under the following optimum conditions; 1: 1 mixture of ethanol (10 %, v/v): n-butanol (30 %, v/v) with a contact time of 10 min at 28±2 °C. |
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| Date |
2015
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Rights |
—
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| Identifier |
http://ir.cftri.com/12097/1/Journal%20of%20Food%20Science%20and%20Technology%20September%202015%2C%20Volume%2052%2C%20Issue%209%2C%20pp%206003-6009.pdf
Gobinath, D. and Prapulla, S. G. (2015) Transgalactosylating β-galactosidase from probiotic Lactobacillus plantarum MCC2156: production and permeabilization for use as whole cell biocatalyst. Journal of Food Science and Technology, 52 (9). pp. 6003-6009. ISSN 0022-1155 |
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