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Characterization of vitamin B12 in Dunaliella salina

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/12124/
http://dx.doi.org/10.1007/s13197-015-2005-y
 
Title Characterization of vitamin B12 in Dunaliella salina
 
Creator Kumudha, Anantharajappa
Sarada, R.
 
Subject 01 Algae
04 Vitamins
 
Description Vitamin B12 is one of nature’s complex metabolite
which is industrially produced using certain bacteria. Algae
could be an alternative source of vitamin B12 and in this study,
vitamin B12 from a halotolerant green alga, Dunaliella salina
V-101 was purified and characterized. The extract of
Dunaliella was purified by passing through Amberlite XAD-
2 and EASI-extract vitamin B12 immunoaffinity column. The
total vitamin B12 content in purified sample fractions was
42 ± 2 μg/100 g dry weight as determined by the chemiluminescence
method which was almost close to 49 ± 2 μg/100 g
dry weight as estimated by microbiological method. Further
quantification of total vitamin B12 using gold nanoparticle
(AUNPs) based aptamer showed 40 ± 0.8/100 g dry weight.
There was a good correlation among all the methods of quantification.
Adenosylcobalamin, a form of vitamin B12 which is
a cofactor for methylmalonyl CoA mutase was identified by
HPLC. Upon quantification, Dunaliella was found to contain
34 ± 4 μg of adenosylcobalamin for 100 g dry biomass.
Authenticity of adenosylcobalmin was confirmed by tandem
mass spectrometry (MS/MS), selected ion recording (SIR) and
multiple reaction monitoring (MRM) studies.
 
Date 2016
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12124/1/Journal%20of%20Food%20Science%20and%20Technology%20January%202016%2C%20Volume%2053%2C%20Issue%201%2C%20pp%20888-894.pdf
Kumudha, Anantharajappa and Sarada, R. (2016) Characterization of vitamin B12 in Dunaliella salina. Journal of Food Science and Technology, 53 (1). pp. 888-894. ISSN 0022-1155