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Brain Polysomes and Poly-U Stimulated Protein Synthesis in Food Restricted Rats.

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Title Brain Polysomes and Poly-U Stimulated Protein Synthesis
in Food Restricted Rats.
 
Creator Van, T. T.
Srivastava, U. S.
Radhakrishnamurty, R.
Thakur, M. L.
 
Subject 07 Enzyme Biochemistry
 
Description Effect of food restriction on (i) polysomal profiles (ii) distribution of 14C-phenylalanine
and 3H-orotate into membrane bound ribosomes, free polysomes and monoribosomes,
(iii) poly-U stimulated incorporation and (iv) the effect of pH-5 enzyme on the
incorporation of phenylalanine by the ribosomes was investigated in the brain of rats
from well (WF) and dietary restricted groups during the period of gestation alone (EI)
and during the period of growth, gestation and lactation (EII). Polysomal profile
showed a considerable lowering in the membrane bound ribosomes of EI and EII groups
and free polysomes of EI group of rat brain compared with WF group. However, the
membrane bound and free polysomes were higher in EII group compared with EI
group. Monoribosomes decreased condiderably in the EII group compared with WF
and EI group. Distribution of 14C-phenylalanine/mg protein was higher in the
membrane bound ribosomes and monoribosomes in the brains of EI and EII groups and
also in the free polysomes in EII group compared with WF group. Distribution of
radioactive orotate/mg RNA was higher in all the three components of polysomal
profile of EI and EII group brain compared with WF group but it was higher only in
the monoribosomes of the EII group compared with EI group. Brain ribosomes from
EI and EII groups responded more actively to the addition of poly-U than WF group.
Synthesis of protein/synthesis of polyphenylalanine ratio (which roughly represents
polysomes/monosomes ratio) showed lower polysomal content in the EII group brain
compared with WF group. WF polysomes when incubated with EI or EII group pH-5
enzyme showed considerable increases in the incorporation compared with WF pH-5
enzymes. EI group polysomes when incubated with EII group pH-5 enzyme showed
remarkable increase in the incorporation compared with WF and EI pH-5 enzyme. EII
polysomes when incubated with any of the three pH-5 enzyme did not show any
change. These results clearly demonstrate that brain polysomes are very sensitive to
dietary restriction no matter at whatever physiological conditions they are exposed to
this restriction.
 
Date 1995
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12140/1/Nutrition%20Research%2C%20Volume%2015%2C%20Issue%205%2C%20May%201995%2C%20Pages%20699-709.pdf
Van, T. T. and Srivastava, U. S. and Radhakrishnamurty, R. and Thakur, M. L. (1995) Brain Polysomes and Poly-U Stimulated Protein Synthesis in Food Restricted Rats. Nutrition Research, 15 (5). pp. 699-709.