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Diffusion, Frowning and Smiling of Low Molecular Weight Protein Bands: A Simple, Rapid and Efficient Solution

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/12149/
http://dx.doi.org/10.1007/s10989-014-9429-5
 
Title Diffusion, Frowning and Smiling of Low Molecular Weight
Protein Bands: A Simple, Rapid and Efficient Solution
 
Creator Rajagopal, Kammara
Chandana, Thimme Gowda
Praveen Kumar, Singh
 
Subject 29 Protein Chemistry
 
Description A modified method of SDS-polyacrylamide gel
electrophoresis for the efficient separation of low molecular
weight proteins (ranging 1.4–10 kDa, containing disulfide
bonds) without frowning, smiling and diffusion is
described. SDS-polyacrylamide and tricine gel electrophoresis
are considered as standard approaches for low
molecular weight protein separation, despite frowning,
smiling and diffusion problems. These methods are inefficient
and confusing as Coomassie brilliant blue stained low
molecular weight protein bands are difficult to identify
since proteins get diffused and, it is difficult to identify as
proteins. The method developed offers a solution to the
existing problem and is more suitable for the efficient
separation of low molecular weight proteins with high
resolution (with di-sulfide bonds).
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/12149/1/International%20Journal%20of%20Peptide%20Research%20and%20Therapeutics%20March%202015%2C%20Volume%2021%2C%20Issue%201%2C%20pp%207-11.pdf
Rajagopal, Kammara and Chandana, Thimme Gowda and Praveen Kumar, Singh (2015) Diffusion, Frowning and Smiling of Low Molecular Weight Protein Bands: A Simple, Rapid and Efficient Solution. International Journal of Peptide Research and Therapeutics, 21 (1). pp. 7-11.