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Isolation and Characterization of Yeasts for Detoxification of Aflatoxin

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Relation http://ir.cftri.com/12169/
 
Title Isolation and Characterization of Yeasts for
Detoxification of Aflatoxin
 
Creator Deepak, M. B.
 
Subject 10 Food Microorganisms
04 Yeast
 
Description The aflatoxins are ubiquitous contaminates of the human food supply throughout the
economically developing world. The adverse toxicological consequences of these
compounds in populations are quite varied owing to a wide range of exposures that
lead to acute effects, including rapid death and chronic outcomes, such as
hepatocellular carcinoma, high hepatitis B virus infection rate etc. To avoid these
problems natural biocontrol strategies need to be implemented to avoid toxicity in
humans and animals. Yeasts / yeast cell wall is the best substances to use
detoxification of aflatoxin. Yeasts, as an inevitable part of the microflora of various
fermented foods and beverages, are found in a wide range of foods from plant or
animal origin, where they have a significant impact on food safety and organoleptic
properties. They provide vitamin B, proteins, peptides, amino-acids and make trace
minerals biological available. Regardless of their non-human origin, such nonpathogenic
yeasts fulfil the major criteria for probiotic definition. Interest in probiotic
yeasts has increased, especially in relation to animal feed but also for human
applications.
The present study is to screen for aflatoxin binding Non Saccharomyces yeast strains.
This is in view to understand, whether food borne strains of yeast species possess
aflatoxin binding properties. This would be of fundamental importance in developing,
understanding and handling of new potential bio-therapeutic agents, or feed additive
for the (functional) food / feed industry. A number of fermented foods were screened
with a view to isolate yeasts having aflatoxin binding capabilities. The promising
yeasts were identified as Pichia manshurica isolate H4S7K13, Saccharomyces
cerevisiae strain Y5-3, Saccharomyces cerevisiae strain MUCL 51248.
Among the isolates non-Saccharomyces Pichia manshurica was found to be effective
in aflatoxin binding and the same organism was fed to wistar albino weanling rats
with and without toxin AFB1 after thirty days. The biochemical parameters indicated
that the isolate Pichia manshurica exhibited similar mycotoxin protective character
like that of Saccharomyces cerevisiae. This indicated that Saccharomyces is not only
organism which can be used for mycotoxin decontamination in food and feed. To our
knowledge this is first report where non-Saccharomyces form have been used for
decontamination of mycotoxin AFB1, and also our earlier experiments proved that
Pichia anomala, Clavispora lusitaniae and Candida tropicalis also have toxin
binding properties. We feel this work will open up similar kind of work and test large
number of organisms, which are part of the fermentation consortium.
 
Contributor Anu Appaiah, K. A.
 
Date 2015
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12169/1/Machettira%20B%20Deepak%20%20Ph.D%20Thesis.pdf
Deepak, M. B. (2015) Isolation and Characterization of Yeasts for Detoxification of Aflatoxin. PhD thesis, University of Mysore.