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Detection, Identification and Characterization of Staphylococci in Street Vend Foods

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/12187/
http://dx.doi.org/10.4236/fns.2011.24044
 
Title Detection, Identification and Characterization of
Staphylococci in Street Vend Foods
 
Creator Rohinishree, Y. S.
Negi, P. S.
 
Subject 10 Food Microorganisms
 
Description In the present investigation the diversity of the Staphylococcus species in different street vend food samples was studied.
A total of 35 staphylococcal food isolates comprising of various species from 14 different street vend food samples were
identified and characterized phenotypically. Staphylococcus aureus was found to be the most prevalent species in these
foods. A PCR-RFLP analysis based on 16S rRNA gene was used for identification of Staphylococcus species. Isolates
showing distinct RFLP pattern for AluI restriction digestion were selected for nucleotide sequence analysis. Phylogenetic
tree constructed using the multiple alignments of 16S rRNA gene sequences of isolates showed a hotspot region of
169 bp and the relationship among species was evaluated by bootstrap values generated in phylogenetic analysis. 16S
rRNA gene sequences allowed bacterial identification that was reproducible and more accurate than that obtained by
phenotypic testing. 16S rRNA gene sequence analysis would be helpful in timely and correct identification of pathogens.
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/12187/1/Food%20and%20Nutrition%20Sciences%2C%202011%2C%202%2C%20304-313.pdf
Rohinishree, Y. S. and Negi, P. S. (2011) Detection, Identification and Characterization of Staphylococci in Street Vend Foods. Food and Nutrition Sciences, 2. pp. 304-313.