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Cost-Effective Endo-Mannanase from Bacillus Sp. Cfr1601 and its Application in Generation of Oligosaccharides from Guar Gum and as Detergent Additive

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Relation http://ir.cftri.com/12188/
http://dx.doi.org/10.1080/10826068.2013.833108
 
Title Cost-Effective Endo-Mannanase from Bacillus Sp. Cfr1601
and its Application in Generation of Oligosaccharides
from Guar Gum and as Detergent Additive
 
Creator Praveen Kumar, Srivastava
Mukesh, Kapoor
 
Subject 05 Enzymes
07 Waste utilization
 
Description The indigenous bacteria Bacillus sp. CFR1601 produced significant levels of endomannanase
when grown on agro-wastes, namely, green gram husk and sunflower oil cake (25.6
IU=mL), used as sole carbon and nitrogen sources, respectively. Under immobilized cell system,
synthetic supports (polyurethane foam, scotch brite, polyester; up to 33.2 IU=mL) were found
marginally superior as compared to natural supports (cotton and silk; up to 28.2 IU=mL) for
endo-mannanase production. Cooperative interactions between L-lysine HCl (0.3% w=v), Tween
60 (0.3% v=v), and sunflower oil cake (3.0% w=v) in central composite design response surface
methodology ameliorated (1.61-fold) endo-mannanase titers to 48.0 IU=mL. Partially purified
endo-mannanase was tested for its ability to produce oligosaccharides from guar gum. These oligosaccharides
were tested in vitro for their ability to promote growth of Lactobacillus plantarum
MTCC 5422 and Lactobacillus salivarius CHS 1E. Results indicated that low-molecular-weight
degraded products from guar gum were (1) able to support the growth of tested strains [increased
O.D600nm up to 2.3-fold and decrease in pH (
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12188/1/Preparative%20Biochemistry%20%26%20Biotechnology%2C%2044%20392%E2%80%93417%2C%202014.pdf
Praveen Kumar, Srivastava and Mukesh, Kapoor (2014) Cost-Effective Endo-Mannanase from Bacillus Sp. Cfr1601 and its Application in Generation of Oligosaccharides from Guar Gum and as Detergent Additive. Preparative Biochemistry and Biotechnology, 44. pp. 392-417.