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Extracellular endo-mannanase from Bacillus sp. CFR1601: Economical production using response surface methodology and downstream processing using aqueous two phase system

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Relation http://ir.cftri.com/12189/
http://dx.doi.org/10.1016/j.fbp.2013.06.006
 
Title Extracellular endo-mannanase from Bacillus sp. CFR1601:
Economical production using response surface methodology
and downstream processing using aqueous two
phase system
 
Creator Praveen Kumar, Srivastava
Mukesh, Kapoor
 
Subject 04 Fermentation Technology
07 Waste utilization
 
Description Bacillus sp. CFR1601, isolated from decaying plant litter, produced an extra-cellular endo-mannanase (198.0 IU/g)
under solid state fermentation (SSF) using defatted coconut residue as the prime solid substrate. In order to
enhance endo-mannanase production, three component, five level central composite design (CCD) of response
surface methodology (RSM) was used. Based on contour plots and variance analysis, optimum conditions for endomannanase
production from Bacillus sp. CFR1601 were attained when defatted coconut residue was supplemented
with sesame oil meal (10.0, w/w), Tween-80 (0.2%, v/v) and inoculated with bacterial cells from log phase (12 h
old; OD600 nm ≈ 3.6). The empirical model developed through RSM brought about 4.04–4.39-fold (800.0–870.0 IU/g)
improvement in endo-mannanase yield as compared to un-optimized growth conditions. Downstream processing
of endo-mannanase from SSF media was carried out for the first time using polyethylene glycol (PEG)/salt aqueous
two phase system (ATPS). ATPS system consisting of a combination of PEG 3350 12.0% (w/w), Na2SO4 12.0% (w/w),
protein load 10.0% (w/w) and pH 5.0 resulted in one-sided partitioning of endo-mannanase towards bottom phase
with 3.8-fold purification and 95.4% recovery. Second stage ATPS with fresh top phase further improved purification
of endo-mannanase to 12.32-fold. Our overall results suggest a cost-effective and integrated process for production
and downstream processing of endo-mannanase.
 
Date 2013
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12189/1/food%20and%20bioproducts%20processing%209%201%20%28%202%200%201%203%20%29%20672%E2%80%93681%20-%20Copy.pdf
Praveen Kumar, Srivastava and Mukesh, Kapoor (2013) Extracellular endo-mannanase from Bacillus sp. CFR1601: Economical production using response surface methodology and downstream processing using aqueous two phase system. Food and Bioproducts Processing, 91. pp. 672-681.