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Inulin supplementation during gestation mitigates acrylamide-induced maternal and fetal brain oxidative dysfunctions and neurotoxicity in rats

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Relation http://ir.cftri.com/12194/
http://dx.doi.org/10.1016/j.ntt.2015.03.003
 
Title Inulin supplementation during gestation mitigates acrylamide-induced
maternal and fetal brain oxidative dysfunctions and neurotoxicity
in rats
 
Creator Gokul, Krishna
Dr., Muralidhara
 
Subject 17 Toxicology
01 Carbohydrates
 
Description Accumulating evidence suggests that the developing brain is more susceptible to a variety of chemicals. Recent
studies have shown a link between the enteric microbiota and brain function. While supplementation of nondigestible
oligosaccharides during pregnancy has been demonstrated to positively influence human health
mediated through stimulation of beneficial microbiota, our understanding on their neuromodulatory propensity
is limited. In the present study, our primary focus was to examine whether supplementation of inulin (a well
known fructan) during gestation can abrogate acrylamide (ACR)-induced oxidative impairments and neurotoxicity
in maternal and fetal brain of rats. Initially, in a dose-determinative study, we recapitulated the impact of
ACR exposure during gestation days (GD 6–19) on gestational parameters, extent of oxidative impairments in
brain (maternal/fetal), cholinergic function and neurotoxicity. Subsequently, pregnant rats orally (gavage)
administered with inulin (IN, 2 g/kg/day in two equal installments) supplements during gestation days (GD
0–19) were exposed to ACR (200 ppm) in drinking water. IN supplements significantly attenuated ACR-induced
changes in exploratory activity (reduced open field exploration) measured on GD 14. Further, IN restored the
placental weights among ACR exposed dams. Analysis of biochemical markers revealed that IN supplements
effectively offset ACR associated oxidative stress not only in the maternal brain, but in the fetal brain as well.
Elevated levels of protein carbonyls in maternal brain regions were completely normalizedwith IN supplements.
More importantly, IN supplements significantly augmented the number of Bifidobacteria in the cecumof ACR rats
which correlatedwell with the neurorestorative effect as evidenced by restored dopamine levels in the maternal
cortex and fetal brain acetylcholinesterase activity among ACR-exposed dams. Further, IN supplements also
conferred significant protection against mitochondrial dysfunction induced by ACR in both milieus. Although
the precise mechanism/s by which IN supplements during pregnancy attenuate ACR induced neurotoxic impact
merits further investigations, we hypothesize that it may mediate through enhanced enteric microbiota and
abrogation of oxidative stress. Further, our study provides an experimental approach to explore the neuroprotective
role of prebiotic oligosaccharides during pregnancy in reducing the adverse impact of developmental
neurotoxicants.
© 2015 Elsevier Inc. All rights reserved.
1. Introduction
Recent evidence has revealed that gut microbiota possesses the
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/12194/1/Neurotoxicology%20and%20Teratology%2049%20%282015%29%2049%E2%80%9358.pdf
Gokul, Krishna and Dr., Muralidhara (2015) Inulin supplementation during gestation mitigates acrylamide-induced maternal and fetal brain oxidative dysfunctions and neurotoxicity in rats. Neurotoxicology and Teratology, 49. pp. 49-58.