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Microarray data analyses of yeast RNA Pol I subunit RPA12 deletion strain

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Relation http://ir.cftri.com/12203/
http://dx.doi.org/10.1016/j.gdata.2016.04.011
 
Title Microarray data analyses of yeast RNA Pol I subunit RPA12 deletion strain
 
Creator Kamlesh Kumar, Yadav
Ram, Rajasekharan
 
Subject 03 Biochemistry & Molecular Biology
19 Yeast
 
Description The ribosomal RNA (rRNA) biosynthesis is the most energy consuming process in all living cells and the majority
of total transcription activity is dedicated for synthesizing rRNA. The cells may adjust the synthesis of rRNA with
the availability of resources. rRNA is mainly synthesized by RNA polymerase I that is composed of 14 subunits.
Deletion of RPA12, 14, 39 and 49 are viable. RPA12 is a very small protein (13.6 kDa), and the amount of protein
in the cells is very high (12,000 molecules per cell), but the role of this protein is unknown in other cellular metabolic
processes (Kulak et al., 2014 [1]). RPA12 consists of two zinc-binding domains and it is required for the termination
of rRNA synthesis (Mullem et al., 2002 [2]). Deletions of RPA12 in Saccharomyces cerevisiae and
Schizosaccharomyces pombe cause a conditional growth defect (Nogi et al., 1993 [3]). In S. pombe, C-terminal deletion
behaves like wild-type (Imazawa et al., 2001 [4]). This prompted us to investigate in detail the physiological
role of RPA12 in S. cerevisiae, we performed the microarray of rpa12Δ strain and deposited into Gene
Expression Omnibus under GSE68731. The analysis of microarray data revealed that the expression of major cellular
metabolism genes is high. The amino acid biosynthesis, nonpolar lipid biosynthesis and glucose metabolic
genes are highly expressed. The analyses also revealed that the rpa12Δ cells have an uncontrolled synthesis of
cell metabolites, so RPA12 could be a master regulator for whole cellular metabolism
 
Date 2016
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12203/1/Genomics%20Data%2C%20Volume%208%2C%20June%202016%2C%20Pages%20104-105.pdf
Kamlesh Kumar, Yadav and Ram, Rajasekharan (2016) Microarray data analyses of yeast RNA Pol I subunit RPA12 deletion strain. Genomics Data, 8. pp. 104-105.