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ROG1 encodes a monoacylglycerol lipase in Saccharomyces cerevisiae

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Relation http://ir.cftri.com/12215/
http://dx.doi.org/10.1016/j.febslet.2014.11.019
 
Title ROG1 encodes a monoacylglycerol lipase in Saccharomyces cerevisiae
 
Creator Varthini, Lakshmanaperumal Vishnu
Selvaraju, Kandasamy.
Malathi, S.
Vasanthi, Nachiappan
 
Subject 11 Lipid Biochemistry
19 Yeast
 
Description Lipid metabolism is extensively studied in Saccharomyces cerevisiae. Here, we report that revertant
of glycogen synthase kinase mutation-1 (Rog1p) possesses monoacylglycerol (MAG) lipase activity in
S. cerevisiae. The lipase activity of Rog1p was confirmed in two ways: through analysis of a strain
with a double deletion of ROG1 and monoglyceride lipase YJU3 (yju3Drog1D) and by site-directed
mutagenesis of the ROG1 lipase motif (GXSXG). Rog1p is localized in both the cytosol and the
nucleus. Overexpression of ROG1 in a ROG1-deficient strain resulted in an accumulation of reactive
oxygen species. These results suggest that Rog1p is a MAG lipase that regulates lipid homeostasis.
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/12215/1/FEBS%20Letters%20589%20%282015%29%2023%E2%80%9330.pdf
Varthini, Lakshmanaperumal Vishnu and Selvaraju, Kandasamy. and Malathi, S. and Vasanthi, Nachiappan (2015) ROG1 encodes a monoacylglycerol lipase in Saccharomyces cerevisiae. FEBS Letters, 589. pp. 23-30.