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Recombinant endo-mannanase (ManB-1601) production using agro-industrial residues: Development of economical medium and application in oil extraction from copra

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/12256/
http://dx.doi.org/10.1016/j.biortech.2016.02.133
 
Title Recombinant endo-mannanase (ManB-1601) production using
agro-industrial residues: Development of economical medium and
application in oil extraction from copra
 
Creator Gaurav Singh, Kaira
Deepesh, Panwar
Mukesh, Kapoor
 
Subject 07 Waste utilization
06 Coconut
 
Description Expression of pRSETA manb-1601 construct in Hi-Control Escherichia coli BL21 (DE3) cells improved
recombinant endo-mannanase (ManB-1601) production by 2.73-fold (1821 ± 100 U/ml). A low-cost,
agro-industrial residue supplemented industrial medium for enhanced and economical production of
ManB-1601 was developed in two mutual phases. Phase-I revealed the potential of various pre- (induction
time: 5 h, induction mode: lactose 0.5 mM) and post-induction [peptone supplementation: 0.94%
(w/v), glycerol 0.123% (v/v)] parameters for enhanced production of ManB-1601 and resulted in 4.61-
fold (8406 ± 400 U/ml) and 2.53-fold (3.30 g/l) higher ManB-1601 and biomass production, respectively.
Under phase-II, economization of phase-I medium was carried out by reducing/replacing costly ingredients
with solubilized-defatted flax seed meal (S-DFSM), which resulted in 3.25-fold (5926 U/ml) higher
ManB-1601 production. Industrial potential of ManB-1601 was shown in oil extraction from copra as
enzyme treatment led to cracks, peeling, fracturing and smoothening of copra, which facilitated higher
(18.75%) oil yield.
 
Date 2016
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12256/1/Bioresource%20Technology%20209%20%282016%29%20220%E2%80%93227.pdf
Gaurav Singh, Kaira and Deepesh, Panwar and Mukesh, Kapoor (2016) Recombinant endo-mannanase (ManB-1601) production using agro-industrial residues: Development of economical medium and application in oil extraction from copra. Bioresource Technology, 209. pp. 220-227.