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Influence of Different Culture Conditions on Yield of Biomass and Value Added Products in Microalgae

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Relation http://ir.cftri.com/12270/
 
Title Influence of Different Culture Conditions on Yield
of Biomass and Value Added Products in Microalgae
 
Creator Sarada, R.
Ranga Rao, A.
Sandesh Kamath, B.
Dayananda, C.
 
Subject 01 Algae
 
Description Microalgae viz., Botryococcus, Haematococcus and Dunaliella, were studied for their adaptability to different culture conditions.
Botryococcus strains of indigenous origin and culture collection centres were studied for their growth, hydrocarbon and lipid profile and
for their biomass yields under different bicarbonate concentrations. Growth of Haematococcus was compared in autotrophic (ambient and
enriched CO
2
atmosphere) and heterotrophic culture conditions. Influence of ammonium salts, urea and commercial fertilizers as nitrogen
source under autotrophic conditions and different amino acids as nitrogen source under heterotrophic culture conditions, was studied on
growth and astaxanthin content of Haematococcus. Influence of different salinities was studied on growth and -Carotene content of
Dunaliella in AS100 and De Walnes media. Results indicated that Botryococcus strains varied in their biomass yields (0.25-2 g L
-1
),
hydrocarbon content (15-60% w/w) and lipid content (15-30%, w/w). An indigenous strain was able to grow at all the tested concentra-
tions of bicarbonate with maximum biomass yield at 0.25 g L
-1
. The growth rate of Haematococcus was higher in heterotrophic medium
with a cell count of 41-44 × 10
4
on 5
th
day after inoculation. The supplementation of autotrophic medium with 2% CO
2
led to a 4-fold
increase in cell count. Use of DAP as nitrogen source showed a 20% increase in yield of encysted biomass. Both DAP and Suphala led to
astaxanthin productivity of 46-48 g L
-1
. Haematococcus was able to utilize all the amino acids at tested concentrations in heterotrophic
culture conditions. Enhanced growth of Dunaliella was observed at 1.0 M NaCl concentration in both the AS 100 and De Walnes media
while 2.0M NaCl in De Walnes medium showed higher -carotene content (45.6 mg g
-1
).
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/12270/1/DBPBMB_6%28SI2%2977-85_2012.pdf
Sarada, R. and Ranga Rao, A. and Sandesh Kamath, B. and Dayananda, C. (2012) Influence of Different Culture Conditions on Yield of Biomass and Value Added Products in Microalgae. Dynamic Biochemistry Process Biotechnology and Molecular Biology, 6. pp. 77-85.