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Improved β-Glucosidase Production From Rhizopus Stolonifer Utilizing Coffee Husk

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Relation http://ir.cftri.com/12272/
 
Title Improved β-Glucosidase Production From Rhizopus Stolonifer Utilizing Coffee Husk
 
Creator Navya, P. N.
Roopali, N. Bhoite
Pushpa, S. Murthy
 
Subject 04 Fermentation Technology
04 Coffee
07 Waste utilization
 
Description β‐glucosidases (EC 3.2.1.21) play an essential role in efficient hydrolysis of cellulosic biomasses. By
hydrolysis of cellobiose, β‐glucosidases relieve inhibiting conditions, and enhances hydrolysis of
cellulose bycellobiohydrolases and endoglucanases.The feasibility of using coffee husk for the
production of β-glucosidase by Rhizopusstoloniferinsolid-state fermentation was evaluated in this
study.The effect of pH (3-7), moisture (40-80%) and fermentation duration (72-168 h) on β-
glucosidaseproduction was investigated using statistical optimization method. A three variable five
level central composite design was used for the study. The partial purification was carried out using
activated charcoal and ammonium sulphate (80%) and the enzyme was characterized to determine
the optimum pH, temperature and the effect of different metal ions and inhibitors on the enzyme
activity.The fermentation variables on optimization revealed maximum β-glucosidase production
atpH 5.2, moisture 53%with fermentation duration of 112 h. The enzyme was purified 1.7 fold using
ammonium sulphate.It had optimum parameters of 60°C temperature, pH 6.0, Km59 mM, and Vmax
78 μmol mg−1min−1 with cellobiose as a substrate. The enzyme activity was furtherenhanced by 1.15
and 1.2 fold in presence of Ca2+ and Mg2+ metal ions. The activity was inhibited in the presence of
SDS, Fe3+ and Zn2+. These results indicated that coffee husk as a sole carbon sourceprovided
necessary nutrients for cell growth and also β-glucosidasesynthesis. To the best of our knowledge,
this is the first report on β-glucosidaseproduction utilizing coffee husk.The present study
demonstrates that Rhizopusstolonifercanefficiently be used to achieveβ-glucosidase production with
an economical and environmentalbenefit in solid-state fermentation.
 
Date 2012
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/12272/1/International%20Journal%20of%20Current%20Research%2C%20Vol.%204%2C%20Issue%2C%2008%2C%20pp.%20123-129%2C%20August%2C%202012.pdf
Navya, P. N. and Roopali, N. Bhoite and Pushpa, S. Murthy (2012) Improved β-Glucosidase Production From Rhizopus Stolonifer Utilizing Coffee Husk. International Journal of Current Research, 8 (4). pp. 123-129.