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Structural elucidation of possible lutein oxidation products mediated through peroxyl radical inducer 2,2 0-Azobis (2-methylpropionamidine) dihydrochloride: Antioxidant and cytotoxic influence of oxidized lutein in HeLa cells

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Relation http://ir.cftri.com/12286/
http://dx.doi.org/10.1016/j.cbi.2013.03.006
 
Title Structural elucidation of possible lutein oxidation products mediated
through peroxyl radical inducer 2,2 0-Azobis (2-methylpropionamidine)
dihydrochloride: Antioxidant and cytotoxic influence of oxidized lutein
in HeLa cells
 
Creator Rangaswamy, Lakshminarayana
Aruna, G.
Sathisha, U. V.
Shylaja, M. Dharmesh
Baskaran, V.
 
Subject 14 Carotenoid Chemistry
32 Antioxidants
 
Description Aim of this study was to elucidate lutein oxidation products mediated through peroxyl radical inducer
2,20-Azobis (2-methylpropionamidine) dihydroc hloride (AAPH) and to study antioxidant and cytotoxic
effect s of oxidized lutein using liposome and HeLa cells. Lutein (20 lmol) with AAPH (5 mM) in liposome’s
was incubated at 37 �C in dark for 3 h, oxidized lutein products were characterized by LC–MS
(APCI+) and studied for their free radical scavenging activity and cytotoxic effects in terms of cell viability,
cellular glutathione, and malondialdehyde leve ls. AAPH mediated lutein fragmented ions were identified
as 551 (M+ + H+-H2O), 391 (M+ + H+ + O2–C22H32O) and 276 (M+ + H+ + O2–C12H20O) and its isomers as 13-
Z lutein, 13-Z zeaxanthin, 13 0-Z zeaxanthin and all-E zeaxanthin. Free radical scavenging activity of oxidized
lutein was higher by 32.7% (IC50, 2.64 lg) than lutein (IC50, 5.28 lg). Oxidized lutein lowered the
lipid peroxid ation (21%), HeLa cells viability (22%) and glutathione levels (32%) than lutein. To conclude,
the oxidized lutein may be highly reactive, since oxidation by AAPH results in peroxyl radical ions, which
can react with conjugated polyene chain of lutein that could lead to higher antiox idant and cytotoxic
effect s on HeLa cells.
 
Date 2013
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12286/1/Chemico-Biological%20Interactions%20203%20%282013%29%20448%E2%80%93455.pdf
Rangaswamy, Lakshminarayana and Aruna, G. and Sathisha, U. V. and Shylaja, M. Dharmesh and Baskaran, V. (2013) Structural elucidation of possible lutein oxidation products mediated through peroxyl radical inducer 2,2 0-Azobis (2-methylpropionamidine) dihydrochloride: Antioxidant and cytotoxic influence of oxidized lutein in HeLa cells. Chemico-Biological Interactions, 203. pp. 448-455.