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ISOLATION, CHARACTERIZATION AND SCREENING OF POTASSIUM-SOLUBILIZING BACTERIA FROM SOILS OF VARANASI

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Title ISOLATION, CHARACTERIZATION AND SCREENING OF POTASSIUM-SOLUBILIZING BACTERIA FROM SOILS OF VARANASI
 
Creator Vijay Singh Meena
 
Contributor Prof. B. R. Maurya
 
Subject Potassium-solubilizing bacteria, isolation, characterization and screening from soils
 
Description Ph. D. Thesis, Soil Science and Agricultural Chemistry
Potassium is released from the slowly or non-exchangeable K pool from the lattice wedge sites of weathered micaceous clay minerals which are selective for K ions. The remaining pool or major pool of K which holds the bulk 90-98% of the total soil K, is held in structure of the primary K bearing minerals soils, K occurs in the form of silicate minerals viz., muscovite, orthoclase, biotite, feldspar, illite, mica (ruby mica, mica powder, mica scrap, mica stone, mica flakes), vermiculite, smectite etc. Total pool of soil K which is extremely complex can be solubilized by the KSB in the available form to the crops. Keeping these in view this work entitled “Isolation, Characterization and Screening of Potassium-Solubilizing Bacteria from Soils of Varanasi” was carried out. A total of 95 rhizospheric soil samples from eight blocks of Varanasi district were collected from dominating crops such as maize (Z. mays), banana (M. paradisiaca), tobacco (N. tabacum), sugarcane (S. officinarum), pigeon pea (C. cajan), and potato (S. tuberosum). All cropped fields were located in Indo-Gangetic Plains of the holy city Varanasi (El 80.71 m [264.80 ft]; Lt25.3333⁰ N; Lg 83.0000⁰ E) which is also known by the names as Banaras/Kashi in the state of Uttar Pradesh, North Central Part of India. Twelve screened isolates used in nethouse pot experiment with maize crop belong to 4 different species of KSB based on 16S phylogeny. They were Agrobacterium tumefaciens strain (58.33%), Rhizobium pusense strain (16.66%), Flavobacterium anhuiense strain (16.66%), and Rhizobium rosettiformans strain (8.33%). The KSB strain OPVS-11 followed by OPVS-6 significantly reduced pH of MAB and K-release dynamics from waste muscovite and biotite. Temperature as 30⁰C, pH 7.6 and 1% NaCl concentration were recorded as suitable for optimum growth of KSB isolates. KSB strains OPVS-10 and 11 showed their significant superiority over rest of the KSB strain on crop growth, yield attributes content and uptake of nutrients with 100% RDK followed by 75 and 50% RDK. The KSB isolates OPVS-11 followed by OPVS-6 significantly enhanced K-release dynamics from both waste muscovite and biotite. Temperature as 30⁰C, pH 7.6 and 1% NaCl concentration were recorded as suitable for optimum growth of KSB isolates. It was observed that efficiency of KSB isolates on growth, yields and K-uptake of maize crop increased with decreasing in doses of potassium. Application of KSB isolates OPVS-11, 10 and 4 emerged out as the most efficient isolates. These isolates performed well with 75% RDK. Application of KSB and recommended levels of K significantly influenced the yield of maize crop and nutrient availability as compared to uninoculated control. The plant growth promoting activities of KSB strains were also enhanced, especially with 75 and 50% RDK. Results indicated that OPVS-7 and OPVS-9 emerged out as efficient acid and alkali-tolerant KSB isolates, respectively. Use of these efficient KSB isolates under favourable conditions certainly will enhance supply of K to crop. However, authentications through multilocation field trails are necessary prior the recommendation of these KSB isolates as Potassic inoculants.
 
Date 2017-01-19T18:58:07Z
2017-01-19T18:58:07Z
2017
 
Type Thesis
 
Identifier Meena, V. S. 2017. Isolation, characterization and screening of potassium-solubilizing bacteria from soils of Varanasi. Ph. D. Thesis, Department of Soil Science and Agricultural Chemistry, Institute of Agricultural Sciences, BHU, Varanasi.
http://krishikosh.egranth.ac.in/handle/1/97222
 
Language en
 
Relation P0158;
 
Format application/pdf
 
Publisher Institute of Agricultural Sciences, Banaras Hindu University, Varanasi