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Studies on In Vitro Selection and Regeneration of Chrysanthemum (Dendranthema grandiflorum Tzelev) Cv. 'Snow Ball' Resistant to the Culture Filtrate of Fusarium oxysporum f. sp. chrysanthemi

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Title Studies on In Vitro Selection and Regeneration of Chrysanthemum (Dendranthema grandiflorum Tzelev) Cv. 'Snow Ball' Resistant to the Culture Filtrate of Fusarium oxysporum f. sp. chrysanthemi
 
Creator JINDAL, NIDHI
 
Contributor SHARMA, S.K.
 
Subject selection, planting, chrysanthemum, regeneration, fungi, biological phenomena, diseases, vegetative propagation, auxins, biological development
chrysanthemum (Dendranthema grandiflorum Tzvelev) ,in vitro selection,regeneration
 
Description ABSTRACT
The reproducible protocol was optimized for in vitro selection and regeneration of
chrysanthemum (Dendranthema grandiflorum Tzvelev) cv. ‘Snow Ball’ resistant to the
culture filtrate of Fusarium oxysporum f.sp .chrysanthemi. The callus was induced on the
medium (MS basal medium + 10 mg/l kinetin + 1 mg/l NAA). Callus was subjected to
different concentrations of culture filtrate of Fusarium oxysporum f.sp. chrysanthemi where
selective dose to culture filtrate was found to be 8 percent on which 4.54 percent cell
survival of chrysanthemum variety ‘Snow Ball’ was recorded. The best medium for callus
differentiation and shoot bud regeneration was (MS basal + 0.2 mg/l BA + 0.1 mg/l NAA +
1 mg/l GA3) on which 66.67 percent calli regenerating shoots was recorded. Percent survival
of the shoots regenerated from selected calli when tested in vitro with spore suspension
recorded was 20 percent.
 
Date 2017-01-19T16:40:10Z
2017-01-19T16:40:10Z
2010
 
Type Thesis
 
Identifier 47265
http://krishikosh.egranth.ac.in/handle/1/97193
 
Language en
 
Format application/pdf