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MOLECULAR CHARACTERIZATION OF CABBAGE (Brassica oleracea L.var. capitata) GENOTYPES USING RAPD

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Title MOLECULAR CHARACTERIZATION OF CABBAGE (Brassica oleracea L.var. capitata) GENOTYPES USING RAPD
 
Creator THAKUR, NISHA
 
Contributor SRIVASTAVA, D.K.
 
Subject cabbages, rapd, dna, genetics, genotypes, polymorphism, vegetables, planting, genes, diseases
cabbage (Brassica oleracea var. capitata),genotypes
 
Description Abstract
The present studies on ‘Molecular characterization of cabbage (Brassica oleracea
var. capitata) genotypes using RAPD was carried out. The aim of this study was to
investigate genetic relationship among different genotypes of cabbage. Sixteen cabbage
genotypes namely, Golden acre, Best of all, BC-79, Giddeon, Green kid, Cabbage
Mangla, General Cabbage, Darl Cabbage, EC-30191, AC-204, No-4, Green Emperor,
Green Europium,KGAT-3, Pusa Drum Head and No-29 were selected for the study. A
total of 17 primers were tried to generate RAPD profile, out of these reproducible
patterns were obtained with 7 primers. A total of 22 bands were obtained out of which all
were polymorphic and 2 were unique bands. The percentage of total polymorphism was
100%. Similarity index was computed based on Jacquard coefficient and used for cluster
analysis based on UPGMA. RAPD technology could be useful for identification of
different accessions as well as accessing the genetic similarity among different accessions
of cabbage. RAPD analysis could also be useful in certification scheme for releasing
certified plant material.
 
Date 2017-01-19T16:25:29Z
2017-01-19T16:25:29Z
2010
 
Type Thesis
 
Identifier 47236
http://krishikosh.egranth.ac.in/handle/1/97185
 
Language en
 
Format application/pdf