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Molecular characterization of a coat protein gene of Potato virus Y infecting tomato in HP

KrishiKosh

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Title Molecular characterization of a coat protein gene of Potato virus Y infecting tomato in HP
 
Creator VERMA, POOJA
 
Contributor BHARDWAJ, S.V.
 
Subject sampling, viruses, genes, nucleotides, potatoes, genetic structures, proteins, acidity, amino acids, biological phenomena
potyviridae,Potato virus Y ,damaging species ,tomatoes,
 
Description ABSTRACT
Potato virus Y is one of the most economically important and damaging species of
family potyviridae. It stands at top of the ladder bringing down the economic returns below
threshold level in tomato. Samples (1-6) showing prominent virus like symptoms were
collected from six different locations of Solan and Shimla districts. PVY was seems to be is
prevalent in Shimla district of H.P. as confirmed by serological detection of Shimla isolates
using DAS- ELISA. cDNA of the virus nucleic acid was synthesized and PCR amplification
gave amplicon of approximately 600bp when specific designed primers for PVYO CP gene
were used, which proved the presence of PVYO in samples. Molecular characterization was
performed by sequencing of CP region of the viral genome. The sequence of Kufri isolate has
a chain of 549 nucleotides and translated nucleotide sequence has 103 amino acids. This
sequence was submitted to Genebank and has been assigned Accession no. GQ891044. In
Kumarsain isolate, amplicon was found to be 465 nucleotides long. The two isolates shared
94-96% similarity at amino acid level. Studies on coat protein gene sequence of different
strain of PVY depicted that the test isolate PVY-Kufri (GQ891044) shared 3-98% and 5-
100% homology at nucleotide and amino acid levels respectively with 107 other previously
reported PVY strains of the world. The sequence alignment analysis revealed high ongoing
evolutionary rate of CP gene of the virus. Three motifs were form in multiple sequence
alignment of CP amino acid sequences. Phylogenetic trees were constructed by NJ and MP
methods. Close phylogeny of test isolate of Kufri with China and Japan suggests the origin of
test strain of PVY in either of these two countries or vice-versa.
 
Date 2017-01-20T12:14:35Z
2017-01-20T12:14:35Z
2009
 
Type Thesis
 
Identifier 47084
http://krishikosh.egranth.ac.in/handle/1/97323
 
Language en