Record Details

ASSESSMENT OF GENETIC DIVERGENCE OF Bacopa monnieri (Linn.) Pennell IN HIMACHAL PRADESH USING MOLECULAR MARKERS

KrishiKosh

View Archive Info
 
 
Field Value
 
Title ASSESSMENT OF GENETIC DIVERGENCE OF Bacopa monnieri (Linn.) Pennell IN HIMACHAL PRADESH USING MOLECULAR MARKERS
 
Creator SHARMA, POORNIMA
 
Contributor SHIRKOT, POONAM
 
Subject genetics, rapd, planting, bacopa, polymorphism, bacopa monnieri, dna, diseases, developmental stages, fruits
Bacopa monnieri (Linn.) Pennell,triterpenoid saponins, sterols, alkaloids and flavonoids
genetics, rapd, planting, bacopa, polymorphism, bacopa monnieri, dna, diseases, developmental stages, fruits
 
Description ABSTRACT
Bacopa monnieri (Linn.) Pennell is a significant medicinal plant species and possesses multiple
medicinal uses as the plant is a rich source of triterpenoid saponins, sterols, alkaloids and flavonoids. The
present investigation was undertaken to evaluate the usefulness of morphological and RAPD markers for
assessing diversity among thirty six accessions belonging to twelve different phytogeographical locations
of Himachal Pradesh.
On the basis of 9 qualitative and 6 quantitative morphological traits investigated, 3 qualitative
descriptors produced significant variation and accessions of site Nehla were found to possess maximum
stem length, leaf breadth, peduncle length and the number of nodes per stem length and thus was
considered as elite accessions. Simple correlation studies revealed maximum positive correlation
between stem length and the number of nodes per stem length. PCA analysis of quantitative traits
revealed two principal components which contributed for 63.98 per cent of total variation. DNA extraction
was carried out using CTAB method (Doyle and Doyle, 1987) which was modified due to presence of
alkaloids and other secondary metabolites. Molecular characterization of 36 accessions was carried out
by RAPD-PCR technology. A total of 32 primers were screened initially and only 17 primers amplified the
genomic DNA successfully. A total of 109 bands were amplified and 77 of them were polymorphic,
depicting 70.64 per cent polymorphism. Similarity coefficient values among the accessions varied from
0.522 to 0.947. Eleven unique bands were produced specific for selected accessions viz. OPC-04650,
OPS-04900, OPS-04300, OPT-02650, OPT-07900, OPV-021200, OPV-061200, OPV-071700, OPV-141300, OPV-
152700 and OPV-171000. On the basis of this study specific markers for quantitative traits were identified.
Unique markers OPS-04900, OPS-04300, OPT-02650 and OPV-021200 were identified for B30 accession of
Nehla site for maximum stem length, OPV-071000 for maximum leaf length in B21 accession of Nauni site,
OPC-04650 for maximum number of flowers in B8 accession of Machiyal site and OPV-061200 for B31
accession of Karganoo site for minimum number of flowers per stem length. On the basis of this study, it
is concluded that Bacopa monnieri possesses moderate polymorphism and diversity in the state of
Himachal Pradesh.
 
Date 2017-01-19T16:36:09Z
2017-01-19T16:36:09Z
2010
 
Type Thesis
 
Identifier 47253
http://krishikosh.egranth.ac.in/handle/1/97190
 
Language en