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Enhanced neuroprotective effect of fish oil in combination with quercetin against 3‐nitropropionic acid induced oxidative stress in rat brain

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Relation http://ir.cftri.com/12290/
http://dx.doi.org/10.1016/j.pnpbp.2012.08.018
 
Title Enhanced neuroprotective effect of fish oil in combination with quercetin against
3‐nitropropionic acid induced oxidative stress in rat brain
 
Creator Denny Joseph, K.M.
Dr., Muralidhara
 
Subject 17 Toxicology
Fish
 
Description While the beneficial effects of fish oil (FO) supplements on the central nervous system function are well
established, few findings have led to the hypothesis that long term n–3 polyunsaturated fatty acid (n−3
PUFA) supplements at higher doses render the membranes more susceptible to lipid peroxidation. Hence recent
studies suggest the use of dietary antioxidants as adjuncts with n−3 fatty acids to effectively improve
the clinical outcome in neurological disorders. In the present investigation, we examined the hypothesis, if
enrichment of FO with quercetin (a natural flavonoid) can provide a higher degree of neuroprotection and
tested the same in a 3-nitropropionic acid (NPA) rat model. Growing male rats administered with NPA
(25 mg/kg bw/d, i.p. 4 days) were provided either with FO (2 mL/kg bw), or Q (25 mg/kg bw) or FO+Q
for 14 days. NPA elicited marked oxidative stress in brain (striatum and cerebellum) as evidenced by significantly
enhanced ROS, malondialdehyde, protein carbonyls and nitric oxide levels. Although varying degree
of protection was evident among FO or Q groups, complete normalization of oxidative markers ensued
only among FO+Q rats. Further, FO+Q combination completely normalized the elevated acetylcholinesterase
activity and protected against NPA‐induced mitochondrial dysfunctions. NPA induced depletion of dopamine
levels was restored among all groups. Interestingly, NPA induced motor deficits were significantly improved
among FO+Q rats. However, further studies are necessary to understand the mechanism/s by which FO enrichment
with Q provides higher degree of protection. Nevertheless, our findings clearly suggest that the use of
natural phytochemicals with moderate doses of FO may provide better neuroprotection and higher therapeutic
advantage in the prevention or treatment of neurodegenerative diseases like Huntington's disease.
 
Date 2013
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12290/1/Progress%20in%20Neuro-Psychopharmacology%20%26%20Biological%20Psychiatry%2040%20%282013%29%2083%E2%80%9392.pdf
Denny Joseph, K.M. and Dr., Muralidhara (2013) Enhanced neuroprotective effect of fish oil in combination with quercetin against 3‐nitropropionic acid induced oxidative stress in rat brain. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 40. pp. 83-92.