Record Details

Safety Assessment of Bacillus licheniformis Me1 Isolated from Milk for Probiotic Application

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Relation http://ir.cftri.com/12296/
http://dx.doi.org/10.1177/1091581812443388
 
Title Safety Assessment of Bacillus licheniformis
Me1 Isolated from Milk for Probiotic
Application
 
Creator Nithya, V.
Muthukumar, S. P.
Prakash, M. Halami
 
Subject 04 Functional foods
27 Dairy products
 
Description In this study, an in vivo toxicological safety assessment of Bacillus licheniformis Me1, a native isolate from milk, was performed. An
acute toxicity study in male albino Wistar rats demonstrated no treatment-related illness or mortality. A 90-day subchronic oral
toxicity study using 2 doses (1.1 � 1010 and 1.1 � 1011 colony-forming unit [CFU]/kg body weight [BW], respectively) failed to
show dose-dependent illness or mortality. Moreover, neither significant differences in serum biochemical and hematological
analyses nor histopathological changes in organs or tissues were found when compared to the control groups. The no-observedadverse-
effect level (NOAEL) was found to be greater than 1.1 � 1011 CFU/kg BW. The in vivo micronucleus assay in mice did not
reveal any signs of genotoxic effect at any of the doses tested. Furthermore, dermal and acute eye irritation tests conducted in
rabbits showed no edema or erythema and ocular lesions. These results suggest that B licheniformis Me1 can be considered safe for
food industry applications.
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12296/1/International%20Journal%20of%20Toxicology%2031%283%29%20228-237.pdf
Nithya, V. and Muthukumar, S. P. and Prakash, M. Halami (2012) Safety Assessment of Bacillus licheniformis Me1 Isolated from Milk for Probiotic Application. International Journal of Toxicology, 31 (3). pp. 228-237.