Record Details

Immunodetection of Bacillus cereus haemolytic enterotoxin (HBL) in food samples

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/12309/
http://dx.doi.org/10.1039/c3ay41737a
 
Title Immunodetection of Bacillus cereus haemolytic
enterotoxin (HBL) in food samples
 
Creator Mandappa, I. M.
Manonmani, H. K.
 
Subject 10 Food Microorganisms
 
Description In recent years food safety has been a global concern. Foodborne pathogens play a key role in food
contamination and affect human health. Bacillus cereus, a foodborne pathogen, causes gastroenteritislike
symptoms, resulting in vomiting and diarrhea. Bacillus cereus produces four different types of
diarrheal toxins. In this paper we describe a quantitative and sensitive Enzyme Linked Immuno Sorbent
Assay (ELISA) method for the detection of one of the diarrheal toxins – haemolytic enterotoxin (HBL) –
using avian antibodies (IgY). The antibody reaction was optimized for different concentrations of antigen
and antibody dilutions. Antigen concentration of 20 ng protein and antibody concentration of 200 ng
protein were observed to be optimum for the reaction. Cross reactivity with other foodborne pathogens
was less than 5% in competitive ELISA. The detection range and the detection limit were 1 mg to 1 pg,
and 250 pg, respectively. The inhibition concentration (IC50) value of this avian antibody was 473.4 ng.
Spiked recovery studies in different food samples showed that the avian antibodies could recognize B.
cereus haemolytic enterotoxin (HBL) in food samples with negligible matrix interference.
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/12309/1/Anal.%20Methods%2C%202014%2C%206%2C%201841.pdf
Mandappa, I. M. and Manonmani, H. K. (2014) Immunodetection of Bacillus cereus haemolytic enterotoxin (HBL) in food samples. Analytical Methods, 6. pp. 1841-1847.