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Prediction of ligand binding site by insilico approach in cold resistant protein isolated from cold resistant mutant of Pseudomonas fluorescen

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Relation http://ir.cftri.com/12317/
http://dx.doi.org/10.1016/j.jmgm.2012.06.012
 
Title Prediction of ligand binding site by insilico approach in cold resistant protein
isolated from cold resistant mutant of Pseudomonas fluorescen
 
Creator Amit, Kumar
Mahejibin, Khan
 
Subject 02 Bacteriology
 
Description Cold shock proteins perform vital functions, such as mRNA masking, coupling of transcription to translation
and developmental timing and regulation, which aids in survival of microbes in cold stress.
Pseudomonas fluorescens is an ecologically important bacterium which helps in plant growth promotion.
Since the cold tolerant mutant of the bacterium is able to grow at the temperature ranges from 30
to 4◦C, it is of interest to study the process of its survival in the extreme temperatures. Therefore, this
study is focused on the three dimensional structure and molecular modeling of cold resistant protein
(CRP) from P. fluorescens to predict its molecular mechanism. Investigating the structure of CRP confirmed
the presence of a conserved domain characteristic of the cold shock domain (CSD) family and a
single nucleotide binding domain. When 3D structure of CRP was compared with the existing cold shock
proteins, major deviations were found in the loop regions connecting the �2–�3, �3–�4 and �4–�5
sheets. Docking studies showed that CRP forms a significant clamp like structure at the substrate binding
cleft which stabilizes the ligand. Therefore, it can be concluded that CRP has a strong affinity for the poly
thymidine (poly T) stretch and can be considered a candidate for transcription regulation.
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12317/1/Journal%20of%20Molecular%20Graphics%20and%20Modelling%2038%20%282012%29%20101%E2%80%93111.pdf
Amit, Kumar and Mahejibin, Khan (2012) Prediction of ligand binding site by insilico approach in cold resistant protein isolated from cold resistant mutant of Pseudomonas fluorescen. Journal of Molecular Graphics and Modelling, 38. pp. 101-111.