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Comparative study on anti‑oxidant and anti‑inflammatory activities of Caesalpinia crista and Centella asiatica leaf extracts

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Relation http://ir.cftri.com/12333/
http://dx.doi.org/10.4103/0975-7406.129172
 
Title Comparative study on anti‑oxidant and
anti‑inflammatory activities of Caesalpinia
crista and Centella asiatica leaf extracts
 
Creator Ramesh, B. N.
Girish, T. K.
Raghavendra, R. H.
Akhilender Naidu, K.
 
Subject 10 Plants
32 Antioxidants
 
Description Amyloidosis, oxidative stress and inflammation have been strongly implicated in neurodegenerative
disorders like Alzheimer’s disease. Traditionally, Caesalpinia crista and Centella asiatica leaf extracts are used to
treat brain related diseases in India. C. crista is used as a mental relaxant drink as well as to treat inflammatory
diseases, whereas C. asiatica is reported to be used to enhance memory and to treat dementia. Objective: The
present study is aimed to understand the anti-oxidant and anti-inflammatory potential of C. asiatica and C. crista
leaf extracts. Materials and Methods: Phenolic acid composition of the aqueous extracts of C. crista and
C. asiatica were separated on a reverse phase C18 column (4.6 x 250 mm) using HPLC system. Antioxidant
properties of the leaf extracts were determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging
assay and the reducing potential assay. The anti-inflammatory activities of aqueous extracts of C. crista and
C. asiatica were studied using 5-lipoxygenase assay. Polymorphonuclear leukocytes (PMNLs) were isolated
from blood by Ficoll-Histopaque density gradient followed by hypotonic lysis of erythrocytes. Results: Gallic,
protocatechuic, gentisic, chlorogenic, caffeic, p-coumaric and ferulic acids were the phenolic acids identified in
C. crista and C. asiatica leaf aqueous extracts. However, gallic acid and ferulic acid contents were much higher
in C. crista compared to C. asiatica. Leaf extracts of C. asiatica and C. crista exhibited antioxidant properties and
inhibited 5-lipoxygenase (anti-inflammatory) in a dose dependent manner. However, leaf extracts of C. crista
had better antioxidant and anti-inflammatory activity compared to that of C. asiatica. The better activity of
C. crista is attributed to high gallic acid and ferulic acid compared to C. asiatica. Conclusions: Thus, the leaf
extract of C. crista can be a potential therapeutic role for Alzheimer’s disease.
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://ir.cftri.com/12333/1/JPharmBioallSci6286-8409348_022009.pdf
Ramesh, B. N. and Girish, T. K. and Raghavendra, R. H. and Akhilender Naidu, K. (2014) Comparative study on anti‑oxidant and anti‑inflammatory activities of Caesalpinia crista and Centella asiatica leaf extracts. Journal of Pharmacy and Bioallied Sciences, 6 (2). pp. 86-91.