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Production of Bioethanol from Fermented Sugars of Sugarcane Bagasse Produced by Lignocellulolytic Enzymes of Exiguobacterium sp. VSG-1

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Relation http://ir.cftri.com/12356/
http://dx.doi.org/10.1007/s12010-013-0366-0
 
Title Production of Bioethanol from Fermented Sugars
of Sugarcane Bagasse Produced by Lignocellulolytic
Enzymes of Exiguobacterium sp. VSG-1
 
Creator Vijayalaxmi, S.
Anu Appaiah, K. A.
 
Subject 04 Fermentation Technology
25 Sugar/Starch/Confectionery
 
Description Exiguobacterium sp. VSG-1 was isolated from the soil sample and characterized for
the production of lignocellulolytic enzymes. Production of these enzymes by the strain VSG-1
was carried out using steam-exploded sugarcane bagasse (SCB) and found to secrete cellulase,
pectinase, mannanase, xylanase, and tannase. The growth and enzyme production were found
to be optimum at pH 9.0 and 37 °C. Upon steam explosion of SCB, the cellulose increased by
42 %, whereas hemicelluloses and lignin decreased by 40 and 62 %, respectively. Enzymatic
hydrolysis of steam-exploded SCB yielded 640 g/l of total sugars. Fermentation of sugars
produced from pretreated SCB was carried out by using Saccharomyces cerevisiae at pH 5.0
and 30 °C. The alcohol produced was calculated and found to be 62.24 g/l corresponding to
78 % of the theoretical yield of ethanol. Hence, the strain VSG-1 has an industrial importance
for the production of fermentable sugars for biofuels.
 
Date 2013
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/12356/1/Appl%20Biochem%20Biotechnol%20%282013%29%20171246%E2%80%93260.pdf
Vijayalaxmi, S. and Anu Appaiah, K. A. (2013) Production of Bioethanol from Fermented Sugars of Sugarcane Bagasse Produced by Lignocellulolytic Enzymes of Exiguobacterium sp. VSG-1. Applied Biochemistry and Biotechnology, 171. pp. 246-260.