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Solid-state fermentation of Jatropha seed cake for optimization of lipase, protease and detoxification of anti-nutrients in Jatropha seed cake using Aspergillus versicolor CJS-98

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Relation http://ir.cftri.com/12366/
http://dx.doi.org/10.1016/j.jbiosc.2013.07.003
 
Title Solid-state fermentation of Jatropha seed cake for optimization of lipase, protease
and detoxification of anti-nutrients in Jatropha seed cake using
Aspergillus versicolor CJS-98
 
Creator Veerabhadrappa, Mohankumar Bavimane
Shivakumar, Sharath Belame
Somashekar, D.
 
Subject 04 Fermentation Technology
33 Nuts
 
Description This study focused on the solid-state fermentation of Jatropha seed cake (JSC), a byproduct generated after biodiesel
production. Presence of anti-nutritional compounds and toxins restricts its application in livestock feed. The disposal of
the JSC is a major environmental problem in the future, due to the generation of huge quantity of JSC after biodiesel
extraction. Hence the JSC was assessed for its suitability as substrate for production and optimization of lipase and
protease from Aspergillus versicolor CJS-98 by solid-state fermentation (SSF). The present study was also focused on the
biodetoxification of anti-nutrients and toxins in JSC. The SSF parameters were optimized for maximum production of
lipase and protease. Under the optimized conditions, the JSC supplemented with maltose and peptone (2%), adjusted to
pH 7.0, moisture content 40%, inoculated with 1 3 107 spores per 5 g cake and incubated at 25�C, produced maximum
lipase, 1288 U/g and protease, 3366 U/g at 96 h. The anti-nutrients like phytic acid (6.08%), tannins (0.37%), trypsin inhibitors
(697.5 TIU/g), cyanogenic glucosides (692.5 mg/100 g), and lectins (0.309 mg/ml), were reduced to 1.70%, 0.23%,
12.5 TIU/g, 560.6 mg/100 g and 0.034 mg/ml respectively. The main toxic compound phorbol esters content in the JSC was
reduced from 0.083% to 0.015% after SSF. Our results indicate that viability of SSF to utilize the huge amount of seed cake
generated after extraction of biodiesel, for production of industrial enzymes and biodetoxification of anti-nutrients,
toxins.
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12366/1/Journal%20of%20Bioscience%20and%20Bioengineering_2014.pdf
Veerabhadrappa, Mohankumar Bavimane and Shivakumar, Sharath Belame and Somashekar, D. (2014) Solid-state fermentation of Jatropha seed cake for optimization of lipase, protease and detoxification of anti-nutrients in Jatropha seed cake using Aspergillus versicolor CJS-98. Journal of Bioscience and Bioengineering, 117 (2). pp. 208-214.