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Effect of spent turmeric on kidney glycoconjugates in streptozotocin-induced diabetic rats

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Relation http://ir.cftri.com/12373/
http://dx.doi.org/10.1186/2251-6581-13-78
 
Title Effect of spent turmeric on kidney glycoconjugates
in streptozotocin-induced diabetic rats
 
Creator Suresh Kumar, G.
Salimath, P. V.
 
Subject 04 Diabetes Mellitus
07 Turmeric
 
Description Background: Curcumin known to have number of medicinal use and masked the fiber containing ukonan like
active polysaccharide in turmeric and its pharmacological effect will be addressed on diabetic nephropathy
particularly the glycoconjugates of extracellular components viz., glycoproteins and glycosaminoglycans - heparan
sulfate (HS).
Methods: Male Wistar rats were maintained on AIN-76 diet containing 10% spent turmeric and were grouped into
control and STZ induced diabetes SFC/TFC and SFD/TFD, respectively. Diabetic status was monitored using blood
and urine, and at the end, harvested kidneys were used to study the amelioration of glycoprotiens (collagen) and
HS by enzymatic digestion, spectrophotometric, hydroxyproline and agarose electrophoretic methods.
Results: In the present study spent turmeric (10%) fed diabetic rats showed improved glomerular filtration rate
(50%), kidney enlargement (60%) and other glycoconjugate metabolism in kidney. Increased collagen content in
diabetic group was observed by hydroxyproline estimation (24%) and periodic acid-Schiff’s (PAS) staining.
Furthermore, elevated activities of enzymes involved in the synthesis and degradation of glycosaminoglycans (GAGs)
were significantly lowered in spent turmeric fed diabetic group. Improvement in total GAGs (43%) and sulfate content
(18%) followed by fractionation of GAGs using specific enzymes led to HS (28%) in the spent turmeric fed diabetic
group, when compared to starch fed diabetic group and was further confirmed by electrophoresis of GAG.
Conclusion: These results clearly indicate beneficial role of spent turmeric in controlling glycoconjugates such as
glycoproteins and heparan sulfate related kidney complications during diabetes.
 
Publisher 2014, :
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/12373/1/Journal%20of%20Diabetes%20%26%20Metabolic%20Disorders%202014%2C%201378.pdf
Suresh Kumar, G. and Salimath, P. V. (2014) Effect of spent turmeric on kidney glycoconjugates in streptozotocin-induced diabetic rats. Journal of Diabetes and Metabolic Disorders, 13.