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Lutein derived fragments exhibit higher antioxidant and anti-inflammatory properties than lutein in lipopolysaccharide induced inflammation in rats

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Relation http://ir.cftri.com/12397/
http://dx.doi.org/10.1039/c4fo00606b
 
Title Lutein derived fragments exhibit higher
antioxidant and anti-inflammatory properties than
lutein in lipopolysaccharide induced inflammation
in rats
 
Creator Nidhi, Bhatiwada
Sharavana, Gurunathan
Ramaprasad, T. R.
Baskaran, V.
 
Subject 23 Vegetables
32 Antioxidants
 
Description In the present study, we appraise the anti-inflammatory efficacy of lutein oxidative degradation derivatives
mediated through UV-irradiation over lutein in counteracting the inflammation induced by lipopolysaccharide
(LPS) in rats (n = 5 per group). UV-irradiated lutein fragments were identified as anhydrolutein
(B, C40H54O), 2,6,6-trimethylcyclohexa-1,4-dienylium (M1, C9H13), (2E,4E,6E,8E)-9-(4-hydroxy-2,6,6-
trimethylcyclohex-1-1en-1-yl)-3,7-dimethylnona-2,4,6,8-tetraen-1-ylium (M2, C20H29O), 4-[(1E,3E,5E,7E)-
3,7,-dimethyldeca-1,3,5,7-tetraen-1-yl]-3,5,5-methylcyclohex-3-en-1-ol (M3, C21H30O) and zeaxanthin
(M4, C40H56O) and its isomers as 13’-Z zeaxanthin, 13’-Z lutein, all-trans zeaxanthin, and 9-Z lutein.
Induction of inflammation by LPS significantly increased the production of nitrites (3.3 fold in the serum
and 2.6 fold in the liver), prostaglandin E2 (26 fold in the serum), and pro-inflammatory cytokines like
tumor necrosis factor-α (6.6 fold in the serum), and interleukin-6 (4.8 fold in the serum). Oxidative derivatives
of lutein, especially M1, M2 and M3, ameliorated acute inflammation in rats by inhibiting the production
of nitrites, malondialdehyde (MDA), PGE2, TNF-α, and IL-6 cytokines more efficiently than lutein
in rats. The anti-inflammatory mechanism of derivatives might be related to the decrease of inflammatory
cytokines and the increase of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase,
glutathione S transferase, glutathione reductase), which would result in the reduction of iNOS,
COX-2 and MDA and subsequently inflammatory responses.
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/12397/1/Food%20%26%20Function%202015.pdf
Nidhi, Bhatiwada and Sharavana, Gurunathan and Ramaprasad, T. R. and Baskaran, V. (2015) Lutein derived fragments exhibit higher antioxidant and anti-inflammatory properties than lutein in lipopolysaccharide induced inflammation in rats. Food and Function. pp. 1-11.