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Attenuated neuroprotective effect of riboflavin under UV-B irradiation via miR-203/c-Jun signaling pathway in vivo and in vitro

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Title Attenuated neuroprotective effect of riboflavin
under UV-B irradiation via miR-203/c-Jun signaling
pathway in vivo and in vitro
 
Creator Amit Kumar, Tripathi
Bhaskar, N.
 
Subject 04 Vitamins
 
Description Background: Riboflavin (RF) or vitamin B2 is known to have neuroprotective effects. In the present study, we report
the attenuation of the neuroprotective effects of RF under UV-B irradiation. Preconditioning of UV-B irradiated
riboflavin (UV-B-RF) showed attenuated neuroprotective effects compared to that of RF in SH-SY5Y neuroblostoma
cell line and primary cortical neurons in vitro and a rat model of cerebral ischemia in vivo.
Results: Results indicated that RF pretreatment significantly inhibited cell death and reduced LDH secretion compared
to that of the UV-B-RF pretreatment in primary cortical neuron cultures subjected to oxygen glucose deprivation
in vitro and cortical brain tissue subjected to ischemic injury in vivo. Further mechanistic studies using cortical neuron
cultures revealed that RF treatment induced increased miR-203 expression which in turn inhibited c-Jun expression
and increased neuronal cell survival. Functional assays clearly demonstrated that the UV-B-RF preconditioning failed to
sustain the increased expression of miR-203 and the decreased levels of c-Jun, mediating the neuroprotective effects of
RF. UV-B irradiation attenuated the neuroprotective effects of RF through modulation of the miR-203/c-Jun signaling
pathway.
Conclusion: Thus, the ability of UV-B to serve as a modulator of this neuroprotective signaling pathway warrants
further studies into its role as a regulator of other cytoprotective/neuroprotective signaling pathways.
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12410/1/Journal%20of%20Biomedical%20Science%202014%2C%202139.pdf
Amit Kumar, Tripathi and Bhaskar, N. (2014) Attenuated neuroprotective effect of riboflavin under UV-B irradiation via miR-203/c-Jun signaling pathway in vivo and in vitro. Journal of Biomedical Science, 21. pp. 39-48.