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Micropropagation for mass multiplication and enriched production of ascorbic acid in tissue culture foliage of roselle (Hibiscus sabdariffa L.)

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Relation http://ir.cftri.com/12418/
http://dx.doi.org/10.1007/s11627-016-9785-2
 
Title Micropropagation for mass multiplication and enriched
production of ascorbic acid in tissue culture foliage of roselle
(Hibiscus sabdariffa L.)
 
Creator Sravan Kumar, Sandopu
Manoj, Prabhakaran
Giridhar, P.
 
Subject 10 Plants
05 Tissue Culture
 
Description An efficient protocol was developed for the
micropropagation of Hibiscus sabdariffa through nodal explants
(auxillary bud cultures) of in vitro seedlings. Nodal
explants were able to induce multiple shoots on Murashige
and Skoog (MS) basal medium supplemented with
2.0 mg L−1 6-benzylaminopurine, 0.5 mg L−1 indole-3-
acetic acid, and 10 μM silver nitrate (AgNO3; 7–8 shoots/
explant) or 20 μg L−1 triacontanol (TRIA; 4–5 shoots/explant).
Medium devoid of AgNO3 that was supplemented with
0.1–0.5 mg L−1 gibberellic acid (GA3) induced the proliferation
of up to two shoots/explant, but supplementation with 1–
5 mg L−1 GA3 induced a single shoot. The highest frequency
of shoot elongation and rooting was obtained on 0.5-strength
MS medium supplemented with 0.1 or 0.5 mg L−1 GA3, and
on this medium, further proliferation of shoots was also evident.
Micropropagated plants were hardened in the greenhouse
and successfully established in soil. Ascorbic acid level
in leaves of micropropagated plants from AgNO3- and TRIAsupplemented
media was highest (2.24 ± 0.14 and
2.18 ± 0.13 mg g−1 fresh weight, respectively), which was
2.8-fold more than normal ex vitro leaves of the same age
(0.79 ± 0.03 mg g−1 fresh weight).
 
Date 2016
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12418/1/In%20Vitro%20Cell.Dev.Biol.%E2%80%94Plant%20%282016%29%2052427%E2%80%93436.pdf
Sravan Kumar, Sandopu and Manoj, Prabhakaran and Giridhar, P. (2016) Micropropagation for mass multiplication and enriched production of ascorbic acid in tissue culture foliage of roselle (Hibiscus sabdariffa L.). In Vitro Cellular and Developmental Biology - Plant, 52. pp. 427-436.