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Oleic acid, hydroxytyrosol and n-3 fatty acids collectively modulate colitis through reduction of oxidative stress and IL-8 synthesis; in vitro and in vivo studies

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Relation http://ir.cftri.com/12426/
http://dx.doi.org/10.1016/j.intimp.2016.03.019
 
Title Oleic acid, hydroxytyrosol and n-3 fatty acids collectively modulate colitis
through reduction of oxidative stress and IL-8 synthesis; in vitro and
in vivo studies
 
Creator Vijay Kumar Reddy, K.
Akhilender Naidu, K.
 
Subject 17 Fatty Acid Chemistry
 
Description Our recent study has demonstrated that mediumchain triglycerides (MCT) and monounsaturated fatty acids potentiate
the beneficial effects of fish oil on risk factors of cardiovascular disease. In the present study, we have investigated
the influence of MCT or olive oil on the protective and mucosal healing ability of fish oil in ulcerative
colitis using cell simulation and animalmodels. Caco-2 cells growninmediumchain fatty acids enriched medium
has exaggerated t-butyl hydroperoxide induced cell damage, GSH depletion, and IL-1β induced IL-8 synthesis,
compared to the cells grown in oleic acid & hydroxytyrosol (OT) enriched medium. Further, combined treatment
of cells with eicosapentaenoic acid, docosahexaenoic acid, and OT has remarkably attenuated the cell damage,
and IL-8 synthesis, compared to individual treatments. To evaluate the effect of these lipid formulations in vivo,
adult Wistar rats were fed diet enriched with high amount of medium chain triglycerides (MCT), virgin olive
oil, or their combination with fish oil. Colitis was induced in rats using dextran sulfate sodium (DSS) for 7days
followed by 10-days of recovery period. Rats ofMCT group exhibit severe disease activity, higher levels of inflammatory
cytokines in the colon compared to the olive oil group. Furthermore, there was persistent body weight
loss, loose stools, higher levels of inflammatory cytokines in the rats ofMCT group, even after DSSwaswithdrawn
fromdrinkingwater. Conversely, fish oil has remarkably attenuated the DSS induced alterations in both MCT and
olive oil diet groups with significantly greater effect in the olive oil group. Thus,MCT increase the susceptibility to
colitis through oxidative damage and IL-8 synthesis in intestinal epithelial cells. The beneficial effects of virgin
olive oil could be partially attributed to hydroxytyrosol. Combined treatment of hydroxytyrosol, oleic acid and
n-3 fatty acids exhibit huge therapeutic benefits in colitis.
 
Date 2016
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12426/1/International%20Immunopharmacology%2035%20%282016%29%2029%E2%80%9342.pdf
Vijay Kumar Reddy, K. and Akhilender Naidu, K. (2016) Oleic acid, hydroxytyrosol and n-3 fatty acids collectively modulate colitis through reduction of oxidative stress and IL-8 synthesis; in vitro and in vivo studies. International Immunopharmacology, 35. pp. 29-42.