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Rapid Clonal Propagation and Stevioside Profiles of Stevia rebaudiana Bertoni.

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Title Rapid Clonal Propagation and Stevioside Profiles
of Stevia rebaudiana Bertoni.
 
Creator Giridhar, P.
Sowmya, K. S.
Ramakrishna, A.
Ravishankar, G. A.
 
Subject 05 Tissue Culture
06 Trees And Shrubs
 
Description Shoot tips of Stevia rebaudiana Bertoni were cultured on Murashige and Skoog (MS) or B5 medium, supplemented with 6-benzyladenine
(BA), indole-3-butyric acid (IBA) or D-naphthalene acetic acid (NAA) in different combinations. A maximum of 28 shoots per shoot tip
explant were produced on B5 medium supplemented with 4.44 μM BA and 0.80 μM NAA. Significantly more shoots could be obtained
from in vitro shoot tips of Stevia by repeating the cycle. Elongation of microshoots was significant on B5 medium devoid of plant growth
regulators. MS medium containing 0.05 μM-0.25 μM gibberellic acid (GA3) led to elongation of shoots up to 10-12 cm in length with 7-8
nodes each. In vitro leaves derived from primary shoots could regenerate 3-4 shoots on the same medium. In vitro rooting of microshoots
(~4 cm in length) was efficient on MS basal medium with shoots 12-13 cm long having 5 nodes and 11-12 roots in one month. After
hardening in micropots for one month 80% of the rooted plants survived. In another study, incorporation of 13.62 μM thidiazuron (TDZ)
in modified MS medium induced 11-12 multiple shoots from shoot tip explants when inoculated in reverse polarity i.e. shoot tips in
downward direction (inverted mode). Both these methods could be useful for mass multiplication of S. rebaudiana. The stevioside content
of both in vitro leaves and hardened three-months-old ex vitro plants was analysed by HPLC. The highest stevioside content (6.72%
steviolbioside and 0.11% rebaudioside-A on a dry weight basis) were found in ex vitro and in vitro leaves, respectively.
 
Date 2010
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/12428/1/IJPDB_4%281%2947-52.pdf
Giridhar, P. and Sowmya, K. S. and Ramakrishna, A. and Ravishankar, G. A. (2010) Rapid Clonal Propagation and Stevioside Profiles of Stevia rebaudiana Bertoni. International Journal of Plant Developmental Biology, 4 (1). pp. 47-52.