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Effect of Different Carbon Sources on the Growth of Alpha-HCH Degrading Pseudomonas Ptm+ Strain

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Relation http://ir.cftri.com/12607/
 
Title Effect of Different Carbon Sources on the Growth of
Alpha-HCH Degrading Pseudomonas Ptm+ Strain
 
Creator Anu Appaiah, K. A.
Karanth, N. G. K.
 
Subject 26 Pesticide Chemistry
 
Description A bacterial isolate capable of degrading alpha-isomer of the insecticide HCH used this
chemical as the sole source of carbon and energy. Pseudomonas (Ptm' strain) produced 10
times more biomass when mannitol was the carbon source and 3 times more biomass with
glucose. Binary mixtures of mannitol nnd alpha·HCH showed simultaneous utilisation of
both the molecules. Pattern of proliferation on HCH and its structural analog inositol was
similar indicating the involvement of common enzyme systems in their breakdown. Utilisation
of alpha-HCH and yeast extract increased when both substrates were added together to the
medium, suggesting some co-factors contained in yeast extract accelerated alpha HCH
degradation in Ptm+ strain, resulting in better growth. Mannitol (11.1 mM) yeast extract
(0.25%) and HCH (68.8 uM) containing medium was suitable for the biomass production of
HCH degrading bacteria.
 
Date 1996
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/12607/1/J.Soil%20Biol%20Ecol%2016%282%29%20103-107%281996%29.pdf
Anu Appaiah, K. A. and Karanth, N. G. K. (1996) Effect of Different Carbon Sources on the Growth of Alpha-HCH Degrading Pseudomonas Ptm+ Strain. Journal of Soil Biology and Ecology, 16 (2). pp. 103-107.