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Simultaneous detoxification and decolorization of molasses spent wash by the immobilized white-rot fungus Flavodon flavus isolated from a marine habitat

DRS at CSIR-National Institute of Oceanography

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Title Simultaneous detoxification and decolorization of molasses spent wash by the immobilized white-rot fungus Flavodon flavus isolated from a marine habitat
 
Creator Raghukumar, C.
Mohandass, C.
Kamat, S.
Shailaja, M.S.
 
Subject Molasses spent wash
white-rot fungus
 
Description The wastewaters of molasses-based alcohol distilleries contain brown colored melanoidin pigments that are one of the major pollutants. We reported decolorization of such intensely brown colored molasses spent wash (MSW) by Flavodon flavus, a white-rot basidiomycete fungus isolated from a marine habitat. We have further attempted to improve the process of decolorization of MSW by this fungus by immobilization. Polyurethane foam-immobilized-fungus decolorized 10% diluted MSW by 60% and 73 % by day 5 and 7 respectively. The immobilized fungus could be effectively used for a minimum of 3 cycles repeatedly to decolorize MSW. Besides decolorization, the fungus also removed the toxicity of MSW. Toxicity bioassay of the fungus-treated molasses spent wash using an estuarine fish Oreochromis mossambicus showed no liver damage in contrast to untreated effluent, which showed moderate liver damage. We detected benzo(a)pyrene, a polycyclic aromatic hydrocarbon (PAH) in the MSW and this appears to be one of the causes of toxicity of the MSW. The concentration of PAH in the MSW decreased by 68% by day 5 on treatment with the fungus. This is the first report where decolorization of MSW is accompanied by simultaneous detoxification and decrease in PAH content of the MSW. A comparison of gel filtration chromatography of MSW, before and after treatment with the immobilized F. flavus showed disappearance of the most of the colored fractions in the fungus-treated MSW. A possible mechanism of decolorization of MSW is via the action of glucose oxidase accompanied by production of hydrogen peroxide that may ultimately act as a bleaching agent.
 
Date 2006-06-28T12:05:50Z
2006-06-28T12:05:50Z
2004
 
Type Journal Article
 
Identifier Enzyme and Microbial Technology, vol. 35, 197?202p.
http://drs.nio.org/drs/handle/2264/173
 
Language en
 
Rights An edited version of this paper was published by Elsevier
 
Format 162400 bytes
application/pdf
 
Publisher Elsevier