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A combined approach of DNA probe and RFLP for family and species identification of larval stages of commercially important aquatic species: A study on the surfclam Spisula solidissima

DRS at CSIR-National Institute of Oceanography

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Title A combined approach of DNA probe and RFLP for family and species identification of larval stages of commercially important aquatic species: A study on the surfclam Spisula solidissima
 
Creator Achuthankutty, C.T.
 
Subject DNA probe
restriction fragment length polymorphism
 
Description This paper deals briefly with a technique developed for the identification of the early stages of veligers of surfclam Spisula solidissima from the larval stages of other common bivalve species using a combination of DNA probe and restriction fragment length polymorphism (RFLP) analysis. An oilgonucleotide sequence designed from the 18S ribosomal RNA gene (nucleotide position 259-276) provided a sensitive probe for the Family Mactridae, to which S. solidissima belongs. DNA of unknown larvae picked from the plankton samples was extracted using Chelex-100. The 600-bp fragment of the 18S rRNA gene was amplified by polymerase chain reaction (PCR). PCR products and controls were tested with the mactrid-specific probe by dot-blot hybridization. The probe hybridized only with controls and larvae of S. solidissima and Mulinia lateralis (The only other member of Family Mactridae present in the study site). Higher percentage of larval amplification were detected by dot-blot hybridization than by agarose gel electrophoresis indicating that colorimetric detection with a probe is more sensitive than ethidium bromide in the visualization of DNA. Restriction enzymes BtsUI and Aval having different restriction sites were used in RFLP analysis to separate the larvae of S. solidissima from those of M. lateralis. It is suggested that a similar approach in fishery biology may be very effective and quick in identification of eggs and larval stages of commercially important species of shell and finfishes in studies pertaining to population dynamics, reproductive behaviour, larval recruitment, resource evaluation and management
 
Date 2006-09-13T05:31:05Z
2006-09-13T05:31:05Z
2003
 
Type Journal Article
 
Identifier Fishing Chimes, vol.23(1), 85-88.p.
http://drs.nio.org/drs/handle/2264/379
 
Language en
 
Format 83319 bytes
application/pdf
 
Publisher Fishing Chimes