Record Details

Cryopreservation studies on the marine diatom Navicula subinflata Grun

DRS at CSIR-National Institute of Oceanography

View Archive Info
 
 
Field Value
 
Title Cryopreservation studies on the marine diatom Navicula subinflata Grun
 
Creator Redekar, P.D.
Wagh, A.B.
 
Subject marine unicellular algae
marine diatom
 
Description Very little work has been done on marine unicellular algae regarding cryopreservation. The present work was, therefore, undertaken to study the effect of different cryoprotectants and cryopreservation on the growth of marine diatom Navicula subinflata. The effect of cryoprotectants such as Ethylene glycol, DMSO, Methanol, Glycerol and Sucrose was studied on Navicula subinflata at 1M, 2M, 4M and 6M concentrations for a period of 30 and 60 minutes to find out the optimum concentration for the growth of diatom for cryopreservation experiment. The optimum concentration of Ethylene glycol, DMSO and Methanol was found to be 4M in 30 minute experiment, while in 60 minute experiment no satisfactory growth was observed and increasing concentration of cryoprotectant decreased the growth. These experiments after cryopreservation revealed that the maximum growth was recorded in Ethylene glycol treated cells for 0 day sample and 7 days and 30 days liquid nitrogen preserved samples. No growth was observed in Methanol (4M) treated cells for 7 days, 15 and 30 days liquid nitrogen preserved samples. Thus the present work reveals the possibility of application of cryopreservation in marine algae.
 
Date 2006-10-17T11:55:59Z
2006-10-17T11:55:59Z
2000
 
Type Journal Article
 
Identifier Seaweed Res. Util., vol.22(1&2), 183-187p.
http://drs.nio.org/drs/handle/2264/442
 
Language en
 
Rights This full-text version of the paper hosted with the permission
 
Format 69142 bytes
application/pdf
 
Publisher Seaweed research and Utilization Association