Record Details

Cell damage and recovery in cryopreserved microphytobenthic diatoms

DRS at CSIR-National Institute of Oceanography

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Title Cell damage and recovery in cryopreserved microphytobenthic diatoms
 
Creator Mitbavkar, S.
Anil, A.C.
 
Subject phytoplankton culture
cell culture
freezing storage
cryobiology
bilogical damage
intertidal environment
Amphora coffeaeformis
Navicula delicatula
 
Description Two pennate microphytobenthic diatoms, Amphora coffeaeformis (Agardh) Kutzing and Navicula transitans var. derasa f. delicatula Heimdal, were cryopreserved and monitored on thawing to track the mechanical injuries and their post-preservation recovery. Cells were subjected to (1) direct freezing in liquid nitrogen and (2) two-step cooling with and without the cryoprotectant, dimethyl sulfoxide (Me2 SO). Mechanical injury due to exposure to low temperature diVered between the two species. While A. coffeaeformis cells were intact and could survive even direct freezing without a cryoprotectant, N. delicatula cell chloroplasts were damaged. However, the two-step cooling along with a cryoprotectant minimized the mechanical injury to cells of both species thereby enhancing the post-thaw viability.
 
Date 2008-02-12T04:35:03Z
2008-02-12T04:35:03Z
2006
 
Type Journal Article
 
Identifier Cryobiology, Vol.53; 143-147p.
http://drs.nio.org/drs/handle/2264/815
 
Language en
 
Rights Copyright [2006]. It is tried to respect the rights of the copyright holders to the best of the knowledge. If it is brought to our notice by copyright holder that the rights are voilated then the item would be withdrawn.
 
Publisher Elsevier