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Extracellular polysaccharide production by Thraustochytrid protists

DRS at CSIR-National Institute of Oceanography

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Title Extracellular polysaccharide production by Thraustochytrid protists
 
Creator Jain, R.
Raghukumar, S.
Tharanathan, R.
Bhosle, N.B.
 
Subject polysaccharides
protists
strains
electron microscopy
light microscopy
proteins
lipids
molecular weight
 
Description Four strains of marine stramenopilan protists, the thraustochytrids, were studied for their ability to produce extracellular polysaccharides (EPSs). Observations by light and scanning electron microscopy revealed the production of a matrix of EPS around groups of cells in stationary cultures. EPS in shake culture filtrates ranged from 0.3 to 1.1 g/L. EPS production, which was studied in greater detail in 2 isolates, SC-1 and CW1, increased with age of cultures, reaching a peak in the stationary phase. Anion exchange chromatography yielded a single fraction of the EPS of both species. The EPS contained 39% to 53% sugars, besides proteins, lipids, uronic acids, and sulfates. Molecular weight of the EPS produced bySC-1 was approximately 94 kDa, and that of CW1, 320 kDa. Glucose formed the major component in the EPS of both isolates-galactose, mannose, and arabinose being the other components. Cultures of both isolates survived air-drying up to a period of 96 hours, suggesting a role for EPS in preventing desiccation of cells.
 
Date 2008-02-22T04:45:42Z
2008-02-22T04:45:42Z
2005
 
Type Journal Article
 
Identifier Marine biotechnology, Vol.7; 184-192p.
http://drs.nio.org/drs/handle/2264/881
 
Language en
 
Rights Copyright [2005]. It is tried to respect the rights of the copyright holders to the best of the knowledge. If it is brought to our notice by copyright holder that the rights are voilated then the item would be withdrawn.
 
Publisher Springer