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Evaluation of an immunodot test to manage white spot syndrome virus (WSSV) during cultivation of the giant tiger shrimp Penaeus monodon

DRS at CSIR-National Institute of Oceanography

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Title Evaluation of an immunodot test to manage white spot syndrome virus (WSSV) during cultivation of the giant tiger shrimp Penaeus monodon
 
Creator Patil, R.
Palaksha, K.J.
Anil, T.M.
Guruchannabasavanna
Patil, P.
Shankar, K.M.
Mohan, C.V.
Sreepada, R.A.
 
Subject polymerase chain reaction
viral diseases
monoclonal antibodies
immunoassays
marine crustaceans
crustacean culture
Penaeus monodon
 
Description A monoclonal antibody-based immunodot test was compared to a polymerase chain reaction (PCR) assay for managing white spot syndrome virus (WSSV) on shrimp farms at Kundapur and Kumta situated in Udupi and Uttar Kannada Districts, respectively, of Karnataka on the west coast of India. Of 12 grow-out farms in Kundapur, 6 (F1 to F6) yielded shrimp samples that were negative for WSSV by both immunodot test and 1-step PCR from stocking to successful harvest. Samples from the other 6 farms (F7 to F12) were positive for WSSV by both immunodot test and 1-step PCR at various times post stocking, and their crops failed. In the 2 farms at Kumta (F13, F14), immunodot and 1-step PCR results were both negative, and harvests were successful. In contrast to 1-step PCR results, farms F5, F6, F13, and F14 gave positive results for WSSV by 2-step PCR, and they were successfully harvested at 105 d post stocking. Our results indicate that an inexpensive immunodot assay can be used to replace the more expensive 1-step PCR assay for disease monitoring.
 
Date 2008-05-01T11:44:37Z
2008-05-01T11:44:37Z
2008
 
Type Journal Article
 
Identifier Diseases Of Aquatic Organisms, Vol.79; 157-161p.
http://drs.nio.org/drs/handle/2264/1062
 
Language en
 
Rights Copyright [2008]. It is tried to respect the rights of the copyright holders to the best of the knowledge. If it is brought to our notice by copyright holder that the rights are voilated then the item would be withdrawn.
 
Publisher Inter Research