Record Details

Role of beta 1-4 linked polymers in the biofilm structure of marine Pseudomonas sp. CE-2 on 304 stainless steel coupons

DRS at CSIR-National Institute of Oceanography

View Archive Info
 
 
Field Value
 
Title Role of beta 1-4 linked polymers in the biofilm structure of marine Pseudomonas sp. CE-2 on 304 stainless steel coupons
 
Creator Jain, A.
Bhosle, N.B.
 
Subject Pseudomonas sp.
b 1-4 linked polymer
biofilms
calcofluor
 
Description Pseudomonas sp CE-2 cells attach and form biofilms on 304-stainless steel (SS) coupons. A series of experiments were carried out in order to understand the role of exopolysaccharides (EPS) in the formation and maintenance of CE-2 biofilms on SS coupons. The biofilm density and EPS concentration increased over the period of incubation and the highest values for both were recorded after 72 h. Calcofluor and the lectin concanavalin A (Con A) showed a positive interaction with 72-h old biofilms, indicating the presence of beta 1-4 linked polymers, and alpha-D-glucose and alpha-D-mannose in the biofilm matrix of CE-2. When the CE-2 cells were grown in the presence of calcofluor (200 mu g ml sup(-1)), biofilm formation was significantly reduced (approx. 85%). Conversely, the lectins Con A or WGA did not influence the CE-2 biofilms on the SS coupons. Furthermore, treatment with cellulase, an enzyme specific for the degradation of beta 1-4 linked polymers, removed substantial amounts of CE-2 biofilm from SS coupons. These results strongly suggest the involvement of beta 1-4 linked polymers in the formation and maintenance of Pseudomonas sp. CE-2 biofilms on SS coupons.
 
Date 2008-07-24T09:31:25Z
2008-07-24T09:31:25Z
2008
 
Type Journal Article
 
Identifier Biofouling, vol.24(4); 283-290p.
http://drs.nio.org/drs/handle/2264/1321
 
Language en
 
Rights This is a preprint of an article whose final and definitive form has been published in the "Biofouling" © 2008 Taylor & Francis; "Biofouling" is available online at http://www.informaworld.com/ with open URL of artilce : http://www.informaworld.com/openurl?genre=article&issn=0892-7014&volume=24&spage=183
 
Publisher Taylor and Francis