Record Details

Opisthorchis viverrini: Detection by polymerase chain reaction (PCR) in human stool samples

DRS at CSIR-National Institute of Oceanography

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Field Value
 
Title Opisthorchis viverrini: Detection by polymerase chain reaction (PCR) in human stool samples
 
Creator Umesha, K.R.
SanathKumar
Parvathi, A.
Duenngai, K.
Sithithaworn, P.
Karunasagar, Indrani
Karunasagar, Iddya
 
Subject microorganisms
polymerase chain reaction
health and safety
defection
 
Description A polymerase chain reaction (PCR) assay was evaluated for detection of Opisthorchis viverrini eggs in the stool specimens of light and heavily infected individuals in Khon Kaen province of Thailand. A total of 75 fecal specimens were analyzed by PCR following DNA extraction. All the microscopically positive samples were positive by PCR, while 23 of 30 (76.6%) microscopically negative samples were also PCR positive. The sensitivity of the assay was 5 eggs/g of stool. This method is potentially useful in the diagnosis of human opisthorchiasis in endemic areas for treatment and in epidemiological investigations.
 
Date 2008-11-25T05:05:16Z
2008-11-25T05:05:16Z
2008
 
Type Journal Article
 
Identifier Experimental Parasitology, vol.120; 353-356p.
http://drs.nio.org/drs/handle/2264/1495
 
Language en
 
Rights Copyright [2008]. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
 
Publisher Elsevier